FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24550
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1226049.93890020366599No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA285911.64562118126273No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC6862.794297352342159No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGCAACTCTGCGT6152.505091649694501No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTTCCGAAAACCAACAAAATAGAAC5732.3340122199592668No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT3461.4093686354378818Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC3061.2464358452138493No Hit
GTGCTGTCTCTCTTTGATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC2120.8635437881873728No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG2080.8472505091649695No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1960.7983706720977597No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA1600.6517311608961304No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT1540.6272912423625255No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1170.47657841140529533Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1120.45621181262729127No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1070.43584521384928715No Hit
GCAAAAGTTCTCAGGGATGTCCTGATCCAACATCGAGGTCATCCCCAAGC1000.40733197556008144No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG910.37067209775967414No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG820.3340122199592668No Hit
GTGCTGTCTCTCTTTGATGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC790.32179226069246436No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA750.30549898167006106No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA720.29327902240325865No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA690.28105906313645623No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC640.2606924643584521Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA580.23625254582484725No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA560.22810590631364563Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAG560.22810590631364563No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTAGTC530.2158859470468432No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG510.20773930753564154No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG490.19959266802443992No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG490.19959266802443992No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT470.1914460285132383No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA470.1914460285132383No Hit
GTGCTGTCTCTCTTTGATGGACTCAGTTCCGAAAACCAACAAAATAGAAC470.1914460285132383No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGGGCTAA460.18737270875763748No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA420.1710794297352342Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAAGTTCTCAGGGATGTGAGGACCACCCCATGTACTCTGCGTTGATA380.15478615071283097No Hit
GTGCTGTCTCTCTTTGAGGGTCATCCCCAAGCAGCTTAGCAACTCTGCGT370.15071283095723012No Hit
GCAAAAGTTCTCAGGGATGTCCGCTGAACTGCTTTACTTGTAGTACCTGC350.14256619144602853No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA350.14256619144602853No Hit
GTGCTGTCTCTCTTTGATGGACCGAGGGCAACGGAGGTCATCCCCAAGCA340.1384928716904277No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG340.1384928716904277No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA330.13441955193482688No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCGGCTAAAGACCAAG330.13441955193482688No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.12219959266802445No Hit
GCAAAAGTTCTCAGGGATGTCGCCGTGAGTTCAGGCTTTTTCATGGTTTA290.11812627291242363No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA290.11812627291242363No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTCATGGTTTAG280.11405295315682282No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.11405295315682282No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA270.109979633401222No Hit
GCAAAAGTTCTCAGGGATGTCGCGTTACCGCACTGGACGCCTCGCGGCGC260.10590631364562118No Hit
GGTGTTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCA250.10183299389002036No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)