FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19254
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT797141.39918977874728No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA232912.096187805131402No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA226411.758595616495274No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA6983.6252207333541087Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT5192.6955437831100033No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC3992.0722966656279214Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC2191.1374259894047989Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA2191.1374259894047989No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1910.9920016619923132Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1590.8258024306637582No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1100.5713098576919082No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT820.42588553027942244No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA740.3843357224472837No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA650.33759218863612755No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA600.3116235587410408No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA540.28046120286693677Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA540.28046120286693677No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT530.2752674768879194No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC500.25968629895086737No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA490.25449257297185No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT460.23891139503479797No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT430.22333021709774592No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA380.19736158720265917No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT370.19216786122364185No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT360.1869741352446245No Hit
CAACCAAAAGAGAGACAGCACCATCAAAGAGAGACAGCACCATCAAAGAG350.18178040926560715No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT330.17139295730757245No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT320.1661992313285551No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG310.16100550534953775No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC300.1558117793705204Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG270.14023060143346838No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA270.14023060143346838No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGATCAAAGA260.13503687545445103No Hit
CAACCAAAAGAGAAGGAGGCCCCATCAAAGAGAGACAGCACCATCAAAGA260.13503687545445103No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.12984314947543368No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA250.12984314947543368No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA240.12464942349641632No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT230.11945569751739898No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG230.11945569751739898No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT230.11945569751739898No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.11426197153838163No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT220.11426197153838163No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG220.11426197153838163No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT200.10387451958034694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)