FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37908
Total Bases5.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA1043427.524533080088638No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA815021.499419647567798No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA383810.124511976363829No Hit
GCAAAAGTTCTCAGGGATGTCCGCAGAGCCAGTCAAGACTTGCCGACAAA23156.106890366149625No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG22075.82199008124934No Hit
GCAAAAGTTCTCAGGGATGTCAGCAGAGCCAGTCAAGACTTGCCGACAAA14563.840877914951989No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC9042.3847209032394217No Hit
GCAAAAGTTCTCAGGGATGTTAGCAGAGCCAGTCAAGACTTGCCGACAAA4311.136963174000211No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA2190.5777144666033556No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT2090.5513348105940699No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1310.34557349372164187No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC1300.3429355281207133No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1210.31919383771235627No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA910.24005486968449932No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA870.22950300728078504No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA770.2031233512714994No Hit
GCAAAGTTCTCAGGGATGTCCGCAGAGCCAGTCAAGACTTGCCGACAAAG760.20048538567057086No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA730.19257148886778516No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT720.1899335232668566No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG720.1899335232668566No Hit
GCAAAAGTTCTAGGGATGTCCGCAGAGCCAGTCAAGACTTGCCGACAAAG690.1820196264640709No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC680.17938166086314233No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG670.17674369526221378No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC650.17146776406035666No Hit
GCAAAAGTTCTCAGGGATGTCCTGGTCCCGCCAATAGAATCCCAATCCTT630.16619183285849953No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT610.1609159016566424No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG590.15563997045478528No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG580.1530020048538567No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT530.13981217684921388No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA480.12662234884457108No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC470.1239843832436425No Hit
GCAAAAGTTCTCAGGGATGTCCATTCCATTCCCCAGGTGCCTCCTTCTCT440.11607048644085682No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGGGCTAA440.11607048644085682No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAC420.11079455523899967No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT420.11079455523899967No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG410.10815658963807112No Hit
GCAAAGTTCTCAGGGATGTCAGCAGAGCCAGTCAAGACTTGCCGACAAAG400.10551862403714254No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA400.10551862403714254No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG400.10551862403714254No Hit
GTGCTGTCTCTCTTTGATGGACCGGTAGAGGCTAAAAGAAAGGCCCAGGT380.10024269283528543No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA380.10024269283528543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)