FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29741
Total Bases4.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1235941.55542853300158No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA571419.212534884502873No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT305310.26529033993477No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA18776.311152953834774No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA7102.3872768232406445No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCTTGGAC2420.8136915369355435No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1470.4942671732624995No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT1400.4707306412023805No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1360.457281194310884No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG830.27907602299855416No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT820.27571366127568003No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT750.252177129215561No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG720.24209004404693854No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA710.23872768232406444No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC680.228640597155442No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT670.2252782354325678No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC660.2219158737096937No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT640.21519115026394536No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG610.20510406509532297No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA550.18492989475807806No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA540.18156753303520393No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA530.17820517131232977No Hit
CAACCAAAAGAGAAGGAGGCACCGAGCCTGGTGCAGTGGTATCAACGCAG520.17484280958945564No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT510.17148044786658148No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA450.15130627752933662No Hit
CAACCAAAAGGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG380.12776974546921757No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT380.12776974546921757No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT370.12440738374634344No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT370.12440738374634344No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT360.12104502202346927No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT360.12104502202346927No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT360.12104502202346927No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT320.10759557513197268No Hit
CAACCAAAAGAGAAGGAGGCTGCTGAGATGGGAAAGGGCTCCTTCAAGTA320.10759557513197268No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG310.10423321340909855No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT310.10423321340909855No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT310.10423321340909855No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG300.10087085168622441No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)