FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11524
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA495342.979868101353695No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC10338.963901423116974No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA6885.970149253731343No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC4353.7747309961818813No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA2752.3863241929885457No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT2111.8309614717112115No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1201.0413051023950017No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAT1080.9371745921555016No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG950.8243665393960431No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA840.7289135716765013No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTATCT820.7115584866365845No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT770.6681707740367928No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT720.6247830614370011Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC620.5380076362374175No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT550.47726483859770913Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAATCAGTTCCGAAAACCAACAAAATAGAAC500.43387712599791733No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG490.42519958347795905No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG480.4165220409580007No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG380.3297466157584172No Hit
GTGCTGTCTCTCTTTGATGGAGAAGAAAGAACTCTGGTGCCTCCTTCTCT380.3297466157584172No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG380.3297466157584172No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC380.3297466157584172No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC380.3297466157584172Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC370.32106907323845885No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG340.29503644567858384No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGGGCTAA310.26900381811870877No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT290.2516487330787921No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA280.24297119055883376Clontech SMART CDS Primer II A (96% over 26bp)
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.22561610551891706No Hit
GTGCTGTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTATCT250.21693856299895867No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.21693856299895867No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA240.20826102047900036No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA230.19958347795904202No Hit
GTGCTGTCTCTCTTTGATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC230.19958347795904202No Hit
GTGCTGTCTCTCTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTATC210.18222839291912532No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG210.18222839291912532No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTA200.17355085039916696No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC200.17355085039916696No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCGGCTAAAGACCAAG180.15619576535925028No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.13884068031933355No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGTCCAAGT150.13016313779937522No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA140.12148559527941688No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA140.12148559527941688No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG140.12148559527941688No Hit
GTGCTGTCTCTCTTTGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC130.11280805275945853No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTACGTTCCCTCCCA130.11280805275945853No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA120.10413051023950018No Hit
GTGCTGTCTCTCTTTGATGGAATCTACCACCCGCTTTGGGCTGCATTCCC120.10413051023950018No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT120.10413051023950018Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGGGCTAA120.10413051023950018No Hit
GTGCTGTCTCTCTTTGATGGAATTTACCACCCGCTTTGGGCTGCATTCCC120.10413051023950018No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG120.10413051023950018No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)