FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27392
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT946134.539281542056074No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT580921.206921728971963No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA301611.01051401869159No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC12294.486711448598131Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA11234.09973714953271Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA3651.3325058411214954No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC3651.3325058411214954Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2380.8688668224299065Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA2190.7995035046728972Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1290.4709404205607477No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1150.41983060747663553No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC880.3212616822429906Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT880.3212616822429906No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA750.27380257009345793No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG710.2591997663551402No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC680.24824766355140185No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG560.20443925233644858No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA520.18983644859813084No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT510.1861857476635514No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG500.18253504672897197No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG420.15332943925233644No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG370.13507593457943926No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG360.1314252336448598No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA340.12412383177570092No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT330.12047313084112149No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT320.11682242990654204No Hit
GCATCATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC320.11682242990654204Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTTGGGGGTGGGGGAGGAGCAAGGGCGTCATCC320.11682242990654204No Hit
GCATCAATCATCACAGGGTTGCACTCGAGAAATCGCGAGATGTCCCTCTT310.1131717289719626No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG310.1131717289719626No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT310.1131717289719626No Hit
GCATCAATCATCACAGGGTACATGGGAGCAGTGGTATCAACGCAGAGTAC300.10952102803738317Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT300.10952102803738317No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA290.10587032710280372No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG290.10587032710280372No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA290.10587032710280372No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA290.10587032710280372No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT280.10221962616822429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)