FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12781
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT512240.07511149362335No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA265120.74172599953055No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA12309.623660120491355No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2481.9403802519364681Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT2041.5961192394961272No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1381.0797277208356155No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1250.9780142398873327No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA1090.8528284171817542No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA610.4772709490650184No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT610.4772709490650184No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA590.461622721226821No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC560.43815037946952506No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT550.4303262655504264No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT510.3990298098740318No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA500.3912056959549331Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCTTGGAC420.3286127846021438No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT410.3207886706830451No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA390.3051404428448478No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA390.3051404428448478No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA380.29731632892574916No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA380.29731632892574916No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC360.28166810108755186Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA290.22689930365386118No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT260.20342696189656523No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGAAC250.19560284797746655No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA250.19560284797746655No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.1877787340583679No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA230.17995462013926922No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT230.17995462013926922No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG220.17213050622017056No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC200.15648227838197323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT200.15648227838197323No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG200.15648227838197323No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC190.14865816446287458No Hit
CAACCAAAAGAGAAGGAGGCCCCATCAAAGAGAGACAGCACCATCAAAGA190.14865816446287458No Hit
CAACCAAAAGAGAAGGAGGCACAGTTGGATGATAGGACAAAAATCACTGA180.14083405054377593No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA180.14083405054377593No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG170.13300993662467728No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGA160.1251858227055786No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT160.1251858227055786No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT160.1251858227055786No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA160.1251858227055786No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT150.11736170878647993No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT150.11736170878647993No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG150.11736170878647993No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT140.10953759486738127No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT140.10953759486738127No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA140.10953759486738127No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGATCAAAGA140.10953759486738127No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT130.10171348094828261No Hit
CAACCAAAAGAGAAGGAGGCACAGTGACGATTCGAGAGATTCTGGAGGAC130.10171348094828261No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)