FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9627
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT476649.50659603199335No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA99510.33551469824452No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA6316.554482185519891No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA2983.0954606834943386No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT1631.6931546691596553No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1541.599667601537343Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1221.2672691388802326Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA580.6024722135660122No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC570.5920847616079775Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT550.5713098576919082No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC520.5401475018178041No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG490.5089851459437No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC400.41549807832138774No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA390.40511062636335304No Hit
GCATCAATCATCACAGGGTTGGGGGTAAGGTCACAGATCAACAGGATCCC350.3635608185312143No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG280.2908486548249714No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA260.27007375090890207No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCTTGGAC250.25968629895086737No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA240.24929884699283264Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA240.24929884699283264No Hit
GCATCAATCATCACAGGGTTGGAGAAGAAGCTGGGAAGGGGACATAGCAA240.24929884699283264No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG210.21813649111872857No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC210.21813649111872857No Hit
GCATCAATCATCACAGGGTTGGGTCTCACTCTCCCTCCTACTCAGCTTCC200.20774903916069387No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG200.20774903916069387No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT190.19736158720265917No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC190.19736158720265917No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA170.1765866832865898Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG160.1661992313285551No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG150.1558117793705204No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT150.1558117793705204No Hit
GCATCAACATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG150.1558117793705204No Hit
GCATCAATCACACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG150.1558117793705204No Hit
GCATCAATCATCACAGGGTTGGGCTCGAGAAATCGCGAGATGTCCCTCTT140.1454243274124857No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.13503687545445103No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.13503687545445103No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT130.13503687545445103No Hit
GCATCAATCATCACAGGGTTGGGGCTAGGGTTACAAATTAACAGCATCTC130.13503687545445103No Hit
CAACCAAAAGAGAAGGAGGCACCGAGCCTGGTGCAGTGGTATCAACGCAG130.13503687545445103No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.13503687545445103No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT120.12464942349641632No Hit
CATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.12464942349641632No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG120.12464942349641632No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG110.11426197153838163No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.11426197153838163No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT100.10387451958034694No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT100.10387451958034694No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAGTCGCGAGATGTCCCTCTT100.10387451958034694No Hit
GCATCAATCATCACAGGGTTGACTCGAGAAATCGCGAGATGTCCCTCTTG100.10387451958034694No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGTGAGATGTCCCTCTT100.10387451958034694No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGATCAAAGA100.10387451958034694No Hit
CAACCAAAAGAGAAGGAGGCTGCTGAGATGGGAAAGGGCTCCTTCAAGTA100.10387451958034694No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)