FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9482
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA423944.70575827884413No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA142315.00738240877452No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC7547.951908879983126No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA1671.7612318076355198No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1551.6346762286437462No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG961.012444631934191No Hit
GCAAAAGTTCTCAGGGATGTCCTCAGCAACCCTGTGATGATTGATGCGGC860.9069816494410462No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG750.7909723686985868No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA650.6855093862054419No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA640.6749630879561274No Hit
GCAAAAGTTCTCAGGGATGTCCTCAGCAACTCTGCGTTGATACCACCCCA550.580046403712297No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA550.580046403712297No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAT480.5062223159670955No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC380.40075933347395065No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC360.37966673697532166No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA340.35857414047669267No Hit
GCAAAAGTTCTCAGGGATGTCCTCAGCAACTCTGCGTTGATACCACTGCT320.3374815439780637Clontech SMART CDS Primer II A (95% over 23bp)
GCAAAAGTTCTCAGGGATGTCCTCAGCAACTCTGCGTTGATACCCCATGT310.3269352457287492No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA300.3163889474794347No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.3163889474794347No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG300.3163889474794347No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA280.2952963509808057No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT250.26365745623286224No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTATAG250.26365745623286224No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG240.2531111579835478No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA240.2531111579835478No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTA230.2425648597342333No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC220.23201856148491878No Hit
GTGCTGTCTCTCTTTGATGGATGCGTGTGGTGCGTGGATATGGATGTGTG200.2109259649862898No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCGGCTAAAGACCAAG180.18983336848766083No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC170.17928707023834634No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC170.17928707023834634No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA160.16874077198903184No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTACAGGGCTCCTGGTGCTGA140.14764817549040285No Hit
GCAAAAGTTCTCAGGGATGTCCTCAGCAACTCTGCGTTGATACCACTGTC130.13710187724108838Clontech SMART CDS Primer II A (95% over 21bp)
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA130.13710187724108838No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGACTCCTGGTGCTGA130.13710187724108838No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.1265555789917739No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT110.11600928074245939No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC110.11600928074245939No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG110.11600928074245939No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG110.11600928074245939No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA110.11600928074245939No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC100.1054629824931449No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGGGCTAAA100.1054629824931449No Hit
GCAAAAGTTCTCAGGGATGTCCTCAGCAACTCTGCGTTGATACCACTCTT100.1054629824931449No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCCCCTGGTGCTGA100.1054629824931449No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCGGCTAAAGACCA100.1054629824931449No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG100.1054629824931449No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)