FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26872
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1154542.962935397439715No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA753128.025454004167905No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA10944.071152128609705No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC7402.7537957725513547No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA1740.6475141411134265No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1670.6214647216433462No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1490.5544805001488539No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1460.5433164632331051No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1340.49866031557011015No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1260.46888955046144687No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1110.41306936588270315No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACTCTGCGTTGATACCACTGCTTC970.3609705269425424Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGAGGCGGCGGTAATGGCGGATGGTGGGTTGT920.34236379874962786No Hit
GTGCTGTCTCTCTTTGATGGACTACAGCTTTGGTGGTTATAGTATTCTTT870.32375707055671327No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC810.30142899672521584No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC680.25305150342363797No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG660.24560881214647215No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT650.24188746650788928No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTAAGC640.23816612086930636No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA630.23444477523072343No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG590.21955939267639177No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG570.21211670139922598No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA550.20467401012206013No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAA540.2009526644834772No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.1600178624590652No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA370.13768978862756773No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACTCTGCGTTGATACCACCCCATG350.1302470973504019No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC350.1302470973504019No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCGGCTAAAGACCAAG340.12652575171181898No Hit
GTGCTGTCTCCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC310.11536171479607026No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACCCTGTGATGATTGATGCGGCTA310.11536171479607026No Hit
GTGCTGTCTCTCTTTGATGGAATGTGGTGGGGGTCTCTTGGGCAGGGAAT290.10791902351890442No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTACTCTGCGTTGAT290.10791902351890442No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC280.10419767788032153No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA270.1004763322417386No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA270.1004763322417386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)