FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18897
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT658534.846801079536434No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA410021.696565592422075No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA17469.239561835211939No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT9985.28126157591152No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA6693.54024448325131No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC2211.1694978038842145Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2181.153622268084881Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGGGTTGGGGGTAAGGTCACAGATCAACAGGATCCC1981.047785362755993No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT1730.9154892310948827No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1240.6561888130391067No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1160.6138540509075515Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1060.5609355982431073No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCTTGGAC810.4286394665819971No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA690.36513732338466426No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG680.3598454781182198No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG600.3175107159866645No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT590.31221887072022014No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC570.3016351801873313No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA540.2857596443879981No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA530.2804677991215537Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT520.2751759538551093No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA500.26459226332222047No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA460.24342488225644285No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT400.21167381065777635No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA390.20638196539133194No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG380.20109012012488756No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG360.19050642959199873No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA330.1746308937926655No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT320.1693390485262211No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA300.15875535799333226Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT300.15875535799333226No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.15346351272688788No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT280.14817166746044344No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG260.13758797692755464No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA220.116420595861777No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.116420595861777No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.116420595861777No Hit
CAACCAAAAGGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG210.1111287505953326No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGAAC210.1111287505953326No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT210.1111287505953326No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)