FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6294
Total Bases910.9 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC100415.951700031776294No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC91314.505878614553543No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA2473.9243724181760404No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT2143.400063552589768No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1933.0664124563075945No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1772.8122020972354624Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1502.3832221163012393Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC1432.2720050842071813No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAG1342.1290117572291067No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA1001.5888147442008262Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC971.5411503018748014Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG761.207499205592628No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA741.1757229107086113No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA590.9374006990784876No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTAGTA580.9215125516364793No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG560.8897362567524627No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA480.7626310772163966Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT470.7467429297743883No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG410.6514140451223388No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA400.6355258976803305No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA400.6355258976803305No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC390.6196377502383222No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT390.6196377502383222No Hit
GTGCTGTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAGTA380.603749602796314No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA380.603749602796314No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG330.5243088655862727No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCCCAT320.5084207181442644No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTA230.36542739116619No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG210.3336510962821735No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCATGT210.3336510962821735No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCGGCTAAAGACCAAG210.3336510962821735No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGGGCTAA200.31776294884016526No Hit
GTGCTGTCTCTCTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAGT200.31776294884016526No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT200.31776294884016526Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC190.301874801398157No Hit
GCAAAAGTTCTCAGGGATGTCCAGGCTTTTTCATGGTTTAGTTCCTCACA190.301874801398157No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC160.2542103590721322No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCAT150.23832221163012393No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA150.23832221163012393No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGAGACCCCCA150.23832221163012393No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCGCCCATGTACTCTGCGTTGATAC150.23832221163012393No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCCATG140.22243406418811568No Hit
GCAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATACC140.22243406418811568No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC140.22243406418811568No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCCATGTACTCTGCGTTGATA130.2065459167461074No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGTCATCCCCAT130.2065459167461074No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGTCCAAGT130.2065459167461074No Hit
GTGCTGTCTCTCTTTGATGGAGGGTGCTGTCTCTCTTTGATGGAGGGTGC120.19065776930409914No Hit
GCAAAAGTTCTCAGGGATGTGAGGCCCACCCATGTACTCTGCGTTGATAC120.19065776930409914No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATCCCCCAT120.19065776930409914No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCATGTA110.17476962186209088No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATGCCCCCA110.17476962186209088No Hit
GTGCTGTCTCTCTTTGATGGACATCCCCAAGCAGCTGCGTTGATACCACT100.15888147442008263No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGTCATCCCCAA100.15888147442008263No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTCATCCCCATGTAC100.15888147442008263No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTCAGGCTTTTTCATGGTTTAG90.14299332697807435No Hit
GCAAAAGTTCTCAGGGATGTCCCCCCATGTACTCTGCGTTGATACCACTG90.14299332697807435Clontech SMART CDS Primer II A (95% over 23bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTGGTCATCCCCATG90.14299332697807435No Hit
GCAAAAGTTCTCAGGGATGTGAGGGCCACCCATGTACTCTGCGTTGATAC90.14299332697807435No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCCCCCATGTACTCTGCGTTGATAC90.14299332697807435No Hit
GTGCTGTCTCTCTTTGATGGACAGCCCCCCCATGTACTCTGCGTTGATAC90.14299332697807435No Hit
GTGCTGTCTCTCTTTGATTGGGCTAAAGACCAAGTCTCTGCTACCGTAGT90.14299332697807435No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACCCCATGTACTCT80.1271051795360661Clontech SMART CDS Primer II A (95% over 21bp)
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGGGCTAAA80.1271051795360661No Hit
GTGCTGTCTCTCTTTGATGATTGATGCGGCTAAAGACCAAGTCTCTGCTA80.1271051795360661No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTATCTC80.1271051795360661No Hit
GTGCTGTCTCTCTTTGATGGACAAGTCACCCAAGCAGGTTGACATCAGGT80.1271051795360661No Hit
GTGCTGTCTCTCTTTGATGGATCCCACTTTCACAAGCTTCCCATGTACTC80.1271051795360661No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC80.1271051795360661No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG80.1271051795360661No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGAGCTGTTTGA80.1271051795360661No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC70.11121703209405784No Hit
GTGCTGTCTCTCTTTGATGGACGGCCCCCCCATGTACTCTGCGTTGATAC70.11121703209405784No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGACCCCCATG70.11121703209405784No Hit
GTGCTGTCTCTCTTTGATGGCTAAAGACCAAGTCTCTGCTACCGTAGTAG70.11121703209405784No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTGGTCATCCCCAAG70.11121703209405784No Hit
GTGCTGTCTCTCTTTGATGCGGCTAAAGACCAAGTCTCTGCTACCGTAGT70.11121703209405784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)