FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6294
Total Bases910.5 kbp
Sequences flagged as poor quality0
Sequence length135-145
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA106616.936765173180806No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC101616.142357801080394Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC74811.88433428662218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA72311.487130600571973Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2884.575786463298379Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT2463.9084842707340326No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA1302.065459167461074No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA1041.6523673339688592Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT921.46170956466476No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA811.2869399428026693No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC771.2233873530346362No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA590.9374006990784876No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC550.8738481093104544No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT450.7149666348903718No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA440.6990784874483635No Hit
CAACCAAAAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA410.6514140451223388No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT380.603749602796314No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC370.5878614553543057Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC320.5084207181442644No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG220.34953924372418177No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGAAC210.3336510962821735No Hit
CAACCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAG210.3336510962821735No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG200.31776294884016526No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCTTGGAC200.31776294884016526No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA180.2859866539561487No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT180.2859866539561487No Hit
CAACCAAAAGAGAGACAGCACCATCAAAGAGAGACAGCACCATCAAAGAG160.2542103590721322No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT150.23832221163012393No Hit
GCATCATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTACA150.23832221163012393Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTTCATGGAAGCAGTGGTATCAACGCAGAGTAC140.22243406418811568Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCCCCATCAAAGAGAGACAGCACCATCAAAGA140.22243406418811568No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGTGGT120.19065776930409914Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGGGTACATGGGAGCAGTGGTATCAACGCAGAGTAC110.17476962186209088Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG110.17476962186209088No Hit
CAACCAAAAGAGAAGGAGGCACCCTCCATCAAAGAGAGACAGCACCCTCC110.17476962186209088No Hit
GCATCATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC110.17476962186209088Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGATCAAAGA100.15888147442008263No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGGA100.15888147442008263Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG100.15888147442008263No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACTTGGACT90.14299332697807435No Hit
CAATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAG90.14299332697807435No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.14299332697807435No Hit
CAACAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCAG90.14299332697807435Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA90.14299332697807435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGCTGC80.1271051795360661No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGCTGCT80.1271051795360661No Hit
GCATCAATCATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTC80.1271051795360661No Hit
GCATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAG80.1271051795360661No Hit
GCATCAATCATCACAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTAC80.1271051795360661Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC70.11121703209405784Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA70.11121703209405784No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACGGCACCATCAAAGA70.11121703209405784No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA70.11121703209405784No Hit
CAACCAAAAGAGAAGGAGGCACTATCAAAGAGAGACAGCACCATCAAAGA70.11121703209405784No Hit
ATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGAT70.11121703209405784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)