FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88702
Total Bases12.8 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC3925544.25492097134225No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA2686130.282293522130278No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA48905.512840747671981No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA11271.2705463236454646No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA9771.1014407792383485No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA5020.5659398886158147No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA4480.505061892629253No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC4370.4926608193727312No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGGGCTAA3870.43629230457035917No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG3550.4002164550968411No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG2880.32468264526166263No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA2850.3213005343735203No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2630.29649838786047666No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2620.2953710175644292No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA2510.28296994430790734No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1910.21532772654506097No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA1750.19728980180830197No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1720.19390769092015964No Hit
GCAAAAGTTCTCAGGGATGTCCTCTTGCTAAGTGGGTGGTTCGCAGAAGC1670.18827083943992243No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1550.17474239588735316No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1430.1612139523347839No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1350.15219498996640438No Hit
GCAAAAGTTCTCAGGGATGTCCCTCATGTCAATCTCATTAAAAGTTGTCA1270.14317602759802484No Hit
GCAAAAGTTCTCAGGGATGTCTACATGGAACTTGTGTGGGTATGGGTCTT1220.13753917611778765No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1220.13753917611778765No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG1220.13753917611778765No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG1140.12852021374940814No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1080.12175599197312349No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCACAGAAGC1040.11724651078893374No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA1030.1161191404928863No Hit
GCAAAAGTTCTCAGGGTGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1000.11273702960474398No Hit
GTGCTGTCTCTCTTTGATGGAATCAGCTCTCTATGTATTGCGCTGTTGGA940.10597280782845934No Hit
GCAAAAGTTCCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA940.10597280782845934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)