FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27293
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1569357.49825962701058No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA411715.08445388927564No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA8613.154655039753783No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4921.8026600227164475Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC3321.2164291210200417No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC3131.1468142014435936No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT2260.8280511486461729No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT2160.7914117172901476No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1530.5605832997471879Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1400.5129520389843549No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT1050.3847140292382662No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA950.3480745978822408No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT810.2967793939838054No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA710.26013996262778005No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA690.25281207635657493No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA670.24548419008536987Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT660.24182024694976734No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA620.2271644744073572No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT610.22350053127175468No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA600.21983658813615214No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT580.21250870186494708No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC560.20518081559374196Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA540.1978529293225369No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT510.1868610999157293No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA500.18319715678012677No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA450.16487744110211408No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT440.16121349796651155No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA430.15754955483090902No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT420.15388561169530648No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA410.15022166855970395No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT410.15022166855970395No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT380.13922983915289636No Hit
CAACCAAAAGAGAAGGAGGCACTCCACTTTTCACACTCAGGAAAACTGGT370.13556589601729382No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT360.1319019528816913No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT340.1245740666104862No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA340.1245740666104862No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGAAC330.12091012347488367No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT330.12091012347488367No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT310.1135822372036786No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT310.1135822372036786No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT300.10991829406807607No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT280.10259040779687098No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT280.10259040779687098No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)