FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27336
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1300047.556335967222715No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA392614.362013462101258No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA9613.515510681884694No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC7462.729002048580626No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC7332.681445712613404No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA3291.203541117939713No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG3081.1267193444541994No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT2951.079163008486977Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG2650.9694176177933861No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT2360.8633304067895815No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1790.6548141644717589No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1530.5597014925373134Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1300.47556335967222707No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC1240.4536142815335089Clontech SMART CDS Primer II A (95% over 24bp)
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1230.4499561018437226No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG1200.4389815627743635No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA1130.413374304945859No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT1130.413374304945859No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC940.34386889083991806No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG880.3219198127011999No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG760.27802165642376353No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA700.25607257828504537No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG680.24875621890547264No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC640.23412350014632718No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG640.23412350014632718No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAACAGCAACTCTG620.22680714076675446No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA580.21217442200760903No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA540.19754170324846357No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAC520.19022534386889084No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTACTC470.17193444541995903No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC470.17193444541995903No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG450.1646180860403863No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG440.16095990635059995No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG410.14998536728124087No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCGGCTAAAGACCAAG370.1353526485220954No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA350.12803628914252269No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA330.12071992976294996Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA320.11706175007316359No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA310.11340357038337723No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.10974539069359088No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.10974539069359088No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC300.10974539069359088No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA290.10608721100380451No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT290.10608721100380451Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT280.10242903131401815No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)