FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49987
Total Bases7.2 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT2159643.20323284053854No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT833316.6703342869146No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA47039.408446196010962No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA19033.8069898173525116Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC6851.3703562926360853Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA6021.204313121411567No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC4810.9622501850481125No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC4250.8502210574749435No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3070.6141596815171945Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC2050.41010662772320805No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT1900.3800988256946806No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1810.36209414447756416No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1810.36209414447756416Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT1750.3500910236661532No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT1660.3320863424490368No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT1520.3040790605557445No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT1420.2840738592033929No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1270.25406605717486547No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA1260.2520655370396303No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1190.2380618960929842No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA1080.2160561746053974No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC970.19405045311781063Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA970.19405045311781063No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA950.1900494128473403No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA910.18204733230639966Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG840.16804369135975353No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT830.1660431712245184No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA810.16204213095404807No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT720.1440374497369316No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT710.14203692960169645No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT620.12403224838457999No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT610.12203172824934484No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA570.11402964770840418No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT540.1080280873026987No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT530.10602756716746355No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT510.10202652689699322No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG500.10002600676175806No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)