FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15415
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA818253.07817061303924No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA195112.656503405773597No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT4512.9257216996432045No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC2151.3947453778786896No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA1891.2260784949724295No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1571.0184884852416478No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA1530.9925397340253No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1500.9730781706130393No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA1500.9730781706130393No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA1060.6876419072332144No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1000.6487187804086928No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA990.6422315926046059No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCTTGGAC940.6097956535841713No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA930.6033084657800843No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT930.6033084657800843No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA930.6033084657800843No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC870.5643853389555628No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA720.46707752189425883Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA710.4605903340901719No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT670.4346415828738242No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGAAC410.26597469996756407No Hit
CAACCAAAAGAGAAGGACAGAGATCCAGAGAGAAGGGGACAGAGATCCAG390.2530003243593902No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG360.23353876094712941No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA350.22705157314304247No Hit
CAACCAAAAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA350.22705157314304247No Hit
CAACCAAAAGAGAAGGAGGCGAGGGAAGGCTTCCAAGGAGAAACAACTTC340.2205643853389556No Hit
CAACCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAG300.19461563412260785No Hit
GGGCAAAGGGTGAGGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA300.19461563412260785No Hit
GGGCAAAGGGTAAAGGGCAAATGAACAAAATGAAAGGAGGCAAGGGCTGC290.1881284463185209No Hit
GGGCAAAGGGTGAAAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA270.17515407071034705No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA270.17515407071034705No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA260.16866688290626014No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG260.16866688290626014No Hit
GGGCAAAGGGTGAAGAGAAAGAGGACAAATGAACAAAATGAAAGGAGGCA250.1621796951021732No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC250.1621796951021732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA240.15569250729808626No Hit
GGGCAAAGGGTGAAGGGCAAATGAACAAAATGAAAGGAGGCAAGGGCTGC230.14920531949399934No Hit
CAACCAAAAGAGAAGGAGGTGAGGGAAGGCTTCCAAGGAGAAACAACTTC220.1427181316899124No Hit
GGGCAAAGGGTGAAGAGAAAGGGGGCAAATGAACAAAATGAAAGGAGGCA210.1362309438858255No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC210.1362309438858255Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA210.1362309438858255No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGATCAAAGA210.1362309438858255No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT210.1362309438858255No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA210.1362309438858255No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG210.1362309438858255No Hit
CAACCAAAAGAGAAGGAGGCAGGAGAGAGTGAACACAGGCATTAAGGGAG190.12325656827765165No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAGTGAAAGGAGGCA190.12325656827765165No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAGATGAACAAAATGAAAGGAGGCA180.11676938047356471No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA180.11676938047356471Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAGACAGCACCATCAAAGAGAGACAGCACCATCAAAGAG170.1102821926694778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)