FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28064
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1356248.32525655644242No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA25439.061431014823262No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC11644.1476624857468645No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACCCCATGT8252.939709236031927No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCTTCC6502.3161345496009123Clontech Universal Primer Mix Long (96% over 25bp)
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCCCATGTACT4971.770952109464082No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA4361.5535917901938427No Hit
GTGCTGTCTCTCTTTGATGGAGCAACTCTGCGTTGATACCACTGCTTCCC3661.3041619156214368Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA3431.2222063854047889No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT2680.9549600912200685No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC1870.6663340935005702No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1640.5843785632839225No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACCCATGTA1590.5665621436716077No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1360.4846066134549601No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1300.46322690992018245No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1180.4204675028506272No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAG1150.4097776510832383No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1010.3598916761687571No Hit
GTGCTGTCTCTCTTTGATGGAGCAACCCTGTGATGATTGATGCGGCTAAA860.306442417331813No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT790.28149942987457244No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG760.2708095781071836No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA740.2636830102622577No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC720.25655644241733183No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCATCCCATGT720.25655644241733183No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTAGAT680.24230330672748004No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG660.23517673888255414No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG650.23161345496009123No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCCCAT580.20667046750285062Clontech SMART CDS Primer II A (95% over 22bp)
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA540.19241733181299886No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT540.19241733181299886No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC500.1781641961231471No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT490.17460091220068413No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA480.17103762827822122No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGTCCCA460.16391106043329534Clontech SMART CDS Primer II A (95% over 21bp)
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA460.16391106043329534No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG420.14965792474344355No Hit
GCAAAAGTTCTCAGGGATGCGAGCAACTCTGCGTTGATACCACCCCATGT410.14609464082098061No Hit
GTGCTGTCTCTCTTTGATGGAGCAACTCTGCGTTGATACCACTGCTTCCA410.14609464082098061Clontech Universal Primer Mix Long (96% over 25bp)
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG400.14253135689851767No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA400.14253135689851767No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA370.13184150513112886No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG370.13184150513112886No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG360.12827822120866592No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCCCAT350.12471493728620296No Hit
GCAAAAGTTCTCAGGGATGCGAGCAACTCTGCGTTGATACCCCATGTACT340.12115165336374002No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA330.11758836944127707No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA330.11758836944127707No Hit
GCAAAAGTTCTCAGGGATGTAAGCAACTCTGCGTTGATACCACTGCTTCC310.11046180159635119Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTA310.11046180159635119No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG310.11046180159635119No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC310.11046180159635119No Hit
GCAAAAGTTCTCAGGGATGCGAGCAACTCTGCGTTGATACCACTGCTTCC290.10333523375142531Clontech Universal Primer Mix Long (96% over 25bp)
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA290.10333523375142531No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)