FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7573
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT492264.9940578370527No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA142218.77723491350852No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT1011.333685461507989No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC710.9375412650204674No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC440.5810114881816981No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC320.42255380958668953No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA310.40934900303710553No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT290.3829393899379374No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.3565297768387693No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT210.277300937541265No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA200.264096130991681No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA190.25089132444209694No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT180.23768651789251288No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA160.21127690479334477No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT160.21127690479334477No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT150.19807209824376074No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA140.18486729169417668No Hit
GGGCAAAGGGTGAAGGGCAAATGAACAAAATGAAAGGAGGCAAGGGCTGC120.1584576785950086No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT120.1584576785950086No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA110.14525287204542453No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT110.14525287204542453No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT110.14525287204542453No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA110.14525287204542453No Hit
TCCTATCAGTGGCCACAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT100.1320480654958405No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA100.1320480654958405No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT90.11884325894625644No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT90.11884325894625644No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA90.11884325894625644No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT90.11884325894625644No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA90.11884325894625644No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCATGGGCTTCACTTACTCT90.11884325894625644No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT80.10563845239667238No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT80.10563845239667238No Hit
CCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT80.10563845239667238No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)