FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820
Total Bases118.7 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT52564.02439024390245No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT455.487804878048781No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA212.5609756097560976No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA202.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC161.951219512195122No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC141.707317073170732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA101.2195121951219512Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA80.975609756097561No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA50.6097560975609756No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.4878048780487805No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT40.4878048780487805No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC30.3658536585365854No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT30.3658536585365854No Hit
GGGCAAAGGGGGGAGAGGCCCTGGGCTTCACTTACTCTTCATTCAAGGCA20.24390243902439024No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACACT20.24390243902439024No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC20.24390243902439024No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT20.24390243902439024No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT20.24390243902439024No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGACCTGGGCTTCACTTACTCT20.24390243902439024No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.24390243902439024No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA20.24390243902439024No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.24390243902439024No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.24390243902439024No Hit
TCCTATCAGTGGTCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.24390243902439024No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT20.24390243902439024No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.24390243902439024No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.24390243902439024No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTT20.24390243902439024Clontech Universal Primer Mix Long (100% over 23bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA20.24390243902439024No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCATGGGCTTCACTTACTCT20.24390243902439024No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC20.24390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT20.24390243902439024No Hit
TCCTATCAGTGGCCGAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.24390243902439024No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACTTGGACTGTA10.12195121951219512No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA10.12195121951219512No Hit
GCATCAATCATCACAGGGTTGGGCTCGAGAAATCGCGAGATGTCCCTCTT10.12195121951219512No Hit
GCATCAATCATCAAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT10.12195121951219512No Hit
GGGTAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA10.12195121951219512No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCTCTGGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTTC10.12195121951219512No Hit
GGGCAAAGGGTGAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGAACC10.12195121951219512No Hit
TCCTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTTC10.12195121951219512No Hit
CAACCAAAAGAGAAGGAAGCAGTGGTATCAACGCAGAGTACATGGCAGCA10.12195121951219512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACTGCACCATCAAAGA10.12195121951219512No Hit
GCATCAATCATCCCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.12195121951219512Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCTATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.12195121951219512Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAAAGAAAGGAGGCA10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT10.12195121951219512No Hit
CATCAAATAGAGAAGGGGCTTGGGCTGTTGATCCATGTGGTGTCTGTAGA10.12195121951219512No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAAGTACA10.12195121951219512No Hit
TCCTATCAGTGGCCGCGTTGCCGACCTCCAGCAGCAGTCGGCTTCTCTAC10.12195121951219512No Hit
CAACCAAAAGAGAAGGAGGAAAAGAGTGTGGACGATCATATTGTACGTTT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCTCTTACTCT10.12195121951219512No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATCGGAAGC10.12195121951219512Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCAGGGCTTCACTTACTCT10.12195121951219512No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACATCACCATCAAAGA10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAAGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
GCATCAATCCTCAAATGGTACATGGGGTGTTATCAACTCAGAGTACATGG10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCTCTGGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGGACGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATGAATGT10.12195121951219512No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTACCAACGCAGAGTA10.12195121951219512Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.12195121951219512No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGAGCTTCACTTACTCT10.12195121951219512No Hit
CAACCAAAAGAGAAGGAGGCACATCAAAGAGAGACAGCACCATCAAAGAG10.12195121951219512No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCACTGGGCTTCACTTACTCTT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCACTGGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTAACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGACCTGGGCTTAACTTACTCT10.12195121951219512No Hit
TCCTATCAGCGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCG10.12195121951219512No Hit
GCATCAATCATCACAGGGTTCATGGAAGCAGTGGTATCAACGCAGAGTAC10.12195121951219512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAGGGTGAAGAGAAAGAGGTCAAATGAACAAAATGAAATGAGGCAA10.12195121951219512No Hit
CCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.12195121951219512No Hit
GCATAAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.12195121951219512Clontech Universal Primer Mix Long (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT10.12195121951219512No Hit
CCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
GCATCAATCATCCAGGGTTATTCCATCAAAGAGAGACAGCACTTGGACTG10.12195121951219512No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.12195121951219512No Hit
TCCTATCAGTGGCCGAAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
GCATCATTCATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTC10.12195121951219512No Hit
CAACCAAAAGGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGAG10.12195121951219512No Hit
GCATCAATCATCGCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT10.12195121951219512No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAAGAGACAGCACTTGGACTGTA10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTAGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACATACTCT10.12195121951219512No Hit
GCATCAATCATCACAGGGTACATGGGAATCAGTGGTATCAACGCAGAGTA10.12195121951219512Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCCTAACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
TACTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
GGGCAAAGGGTGACAGCACGTTACTTGGAGTTGGACTGTAGAACCATGTC10.12195121951219512No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT10.12195121951219512No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACGTACTCT10.12195121951219512No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCACTTACTCTT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCAATTACTCT10.12195121951219512No Hit
GCATAATCATCACAGGGTTGCTCCATCAAAGAGAGACAGCACTTGGACTG10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCCCTTACTCT10.12195121951219512No Hit
GCATCAATCTCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.12195121951219512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.12195121951219512No Hit
TCCTATCAGTGGCCCCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
GGGCAAAGGGTGAAGAGAAAGACAGCACCGTTACTTGGGTTGGACTGTAG10.12195121951219512No Hit
TCCTTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGTGGCACTGGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAATAGGCGAGCGGCCCTGGGCTAAAATTACACT10.12195121951219512No Hit
GCATCAATCATCACAGGGTTGCTCCATCAAAGAGAGACAGCACTTGGACT10.12195121951219512No Hit
GCATCAACATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.12195121951219512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACGTTCTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGCGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCGCTT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.12195121951219512No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA10.12195121951219512No Hit
GCATCAATCATCTCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGATGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGTAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCCTGTCGCCCATCATGGCAAGCACAGAGTTGGATG10.12195121951219512No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.12195121951219512Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGCCCGCAAAGAGGGGAGAGGCCGTGGGCTTCACGTACTCT10.12195121951219512No Hit
TCCTACCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.12195121951219512No Hit
TCCTATCAGTGGCAGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.12195121951219512No Hit
CAACCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAG10.12195121951219512No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACACACAGTAC10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGCAGAGCACCTGGGCTTCACTTACTCT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTACACTTACTCT10.12195121951219512No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTAT10.12195121951219512No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCTTGGGCTTCACTTACTCT10.12195121951219512No Hit
CAACCAAAAGAGAAGGAGGCAGGCGCAACTGCTGGGAAACGCAAAGCATC10.12195121951219512No Hit

[WARN]Adapter Content

Adapter graph