FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19663
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC725136.87636678024717No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA631432.11107155571378No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG11886.041804404210955No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA11235.711234297919951No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA5923.0107308142196003No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1820.9255962976148095No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1530.7781111732695927No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA740.37634135177745004No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA740.37634135177745004No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG700.355998576005696No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG660.3356558002339419No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC640.3254844123480649No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG510.2593703910898642No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA500.2542846971469257No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG490.2491990032039872No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG360.1830849819457865No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT330.16782790011697096No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT320.16274220617403246No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA320.16274220617403246No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT310.15765651223109395No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG310.15765651223109395No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.15257081828815544No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGGGCTAA290.1474851243452169No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG290.1474851243452169No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA290.1474851243452169No Hit
GCAAAAGTTCTCAGGGATGTCCTCTTGCTAAGTGGGTGGTTCGCAGAAGC260.13222804251640136No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.13222804251640136No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.12714234857346285No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA250.12714234857346285No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA240.12205665463052434No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA240.12205665463052434No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA220.11188526674464731No Hit
GCAAAAGTTCCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA210.10679957280170879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)