FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181
Total Bases171.1 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT62753.09060118543607No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA23820.152413209144793No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA352.963590177815411Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA201.6934801016088061No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC141.1854360711261642Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC141.1854360711261642Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC110.9314140558848433No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC90.7620660457239627No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA80.6773920406435224Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT60.5080440304826418No Hit
CAACCAAAAGAGAAGGAGGCACATGTGGGTTCTGCAGAGAGGCAGATCCC60.5080440304826418No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA60.5080440304826418No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC50.42337002540220153No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA50.42337002540220153No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA40.3386960203217612No Hit
TCCTATCAGTGGCCGCAAAGAAGGGAGAGGCCCTGGGCTTCACTTACTCT30.2540220152413209No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA30.2540220152413209No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT30.2540220152413209No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.2540220152413209No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.2540220152413209No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT30.2540220152413209No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.2540220152413209No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA30.2540220152413209No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA30.2540220152413209No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA30.2540220152413209No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.2540220152413209No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA20.1693480101608806No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGTCCCTGGGCTTCACTTACTCT20.1693480101608806No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGGAAGGAGGCA20.1693480101608806No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA20.1693480101608806Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT20.1693480101608806No Hit
TCCTATCAGAGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.1693480101608806No Hit
TCCTGTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.1693480101608806No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.1693480101608806No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.1693480101608806No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTCCTCT20.1693480101608806No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT20.1693480101608806No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA20.1693480101608806No Hit
GCATCAATCATCACAGGGTACATGGGAATCAGTGGTATCAACGCAGAGTA20.1693480101608806Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCCAATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.1693480101608806No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGCGGCCCTGGGCTTCACTTACTCT20.1693480101608806No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT20.1693480101608806No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT20.1693480101608806No Hit
TCCTATCAGTGGCCGCTAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.1693480101608806No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCC20.1693480101608806No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA20.1693480101608806No Hit
GCATCAATCATCACAGGGTTCATGGGAAGCAGTGGTATCAACGCAGAGTA20.1693480101608806Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA20.1693480101608806No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT20.1693480101608806No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTACACTTACTCT20.1693480101608806No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCATCACTTACTCT20.1693480101608806No Hit
GCATCAATATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.1693480101608806Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)