FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16509
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA866752.49863710703253No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT193911.745108728572294No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA11997.262705191107881No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA10516.366224483614998No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC5973.6162093403598035No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA1821.1024289781331396No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1160.7026470410079351No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1000.6057302077654613No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG930.563329093221879No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG820.4966987703676783No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG780.47246956205705976No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG510.3089224059603852No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAT500.30286510388273064No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG420.25440668726149374No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA390.23623478102852988No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG390.23623478102852988No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC380.2301774789508753No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG350.21200557271791143No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA290.17566176025198377No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.16960445817432918No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTATTA270.16354715609667453No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT270.16354715609667453No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA240.1453752498637107No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGGGCTAA230.13931794778605608No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA230.13931794778605608No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.1332606457084015No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG200.12114604155309225No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA170.10297413532012842No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA170.10297413532012842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)