FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3883
Total Bases562.8 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA219756.57996394540304No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA3308.498583569405099No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA1604.120525366984291No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1152.961627607519959No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA461.1846510430079835No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT350.9013649240278137No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCACTGCTTCC340.8756116404841618Clontech Universal Primer Mix Long (96% over 25bp)
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC290.7468452227659027No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.6438320885912954No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.6180788050476436No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG240.6180788050476436No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT210.5408189544166881No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.5150656708730363No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC180.46355910378573273No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCCCATGTACT170.4378058202420809No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG170.4378058202420809No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG150.38629925315477726No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG150.38629925315477726No Hit
GCAAAAGTTCTCAGGGATGTCCGCAACTCTGCGTTGATACCACTGCTTCC140.36054596961112545Clontech Universal Primer Mix Long (96% over 25bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.3347926860674736No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA120.3090394025238218No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC110.28328611898017No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAC100.25753283543651817No Hit
GCAAAAGTTCTCAGGGATGTCCGCAACCCTGTGATGATTGATGCGGCTAA90.23177955189286636No Hit
GCAAAAGTTCTCAGGGATGTCCGCAACTCTGCGTTGATACCCCATGTACT90.23177955189286636No Hit
GCAAAAGTTCTCAGGGATGATCAGCAACTCTGCGTTGATACCACTGCTTC90.23177955189286636Clontech SMART CDS Primer II A (95% over 24bp)
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG90.23177955189286636No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA80.20602626834921456No Hit
GCAAAAGTTCTCAGGGATGGATCAGCAACTCTGCGTTGATACCACTGCTT80.20602626834921456Clontech SMART CDS Primer II A (95% over 24bp)
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCACCCCATGT80.20602626834921456No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTACCA80.20602626834921456No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA80.20602626834921456No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA70.18027298480556272No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA70.18027298480556272No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA70.18027298480556272No Hit
GCAAAAGTTCTCAGGGATGTCCGCAACTCTGCGTTGATACCACCCCATGT70.18027298480556272No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGCGGTTCGCAGAAGCAGCA70.18027298480556272No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT60.1545197012619109No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA60.1545197012619109No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG60.1545197012619109No Hit
GGTGTTTGGGATGTAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA50.12876641771825909No Hit
GGTGTTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCA50.12876641771825909No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG50.12876641771825909No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.12876641771825909No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCGGCTAAAGACCAAG50.12876641771825909No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT40.10301313417460728Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTTGGGATGGAATGGTGATCCACGTAGGTGGTTCGCAGAAGCAGCA40.10301313417460728No Hit
GGTGTTTGGGATGGAATGGTGATCCACACAGGTGGTTCGCAGAAGCAGCA40.10301313417460728No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT40.10301313417460728Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTCCGCAGAAGCAGCA40.10301313417460728No Hit
GTGCTGTCTCTCTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTACC40.10301313417460728No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA40.10301313417460728No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC40.10301313417460728No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)