FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52014
Total Bases7.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1700932.700811320029224No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT1023519.677394547621795No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA759414.599915407390318No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA656812.62736955435075No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC3270.628676894682201No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC2580.4960203022263237No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC2180.419117929788134No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA1630.31337716768562307No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1540.2960741338870304No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT1500.28838389664321146No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG1260.24224247318029762No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1120.2153266428269312No Hit
GCATCAATCATCACAGGGTTGGATTCTCTCTCAGGAGCCACACACTGGGC1110.21340408351597645No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCTTGGAC1100.2114815242050217No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT1070.2057138462721575No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1060.20379128696120274No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1020.19610104971738376No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA960.18456569385165533No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA880.16918521936401737No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA830.15957242280924366No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG830.15957242280924366No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA810.1557273041873342No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT790.15188218556542468No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA780.14995962625446996No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA770.1480370669435152No Hit
CAACCAAAAGAGAAGGAGGCACTGCCTCCACTACAGCAACTGCACCCACG750.14419194832160573No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT730.14034682969969622No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT690.13265659245587727No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA640.12304379590110355No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT620.11919867727919406No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT620.11919867727919406No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA600.11535355865728458No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT570.10958588072442034No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG560.1076633214134656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)