FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71958
Total Bases10.4 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC2791738.79624225242503No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGAAGCAGCAGTGAAGA1856225.795602990633427No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA890212.37110536701965No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA30054.176047138608633No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA17492.4305845076294506No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA7371.0242085661080074No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA5620.7810111453903666No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGAAGCAGCAGTGAAGAG4930.6851218766502681No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGAAGCAGCAGTGAAGAG4670.6489896884293616No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA3480.4836154423413658No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGAAGCAGCAGTGAAGAG2570.3571527835681926No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA2420.33630729036382334No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGAAGCAGCAGTGAAGAG1710.23763862252980905No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1430.1987270352149865No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1430.1987270352149865No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1320.1834403401984491No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT1270.17649184246365934No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1170.1625948469940799No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1140.15842574835320605No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1010.1403596542427527No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGAAGCAGCAGTGAAGAG990.13758025514883682No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA900.12507295922621528No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGAAGCAGCAGTGAAGAG880.12229356013229939No Hit
GCAAAAGTTCCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA750.10422746602184607No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA740.10283776647488814No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGAAGCAGCAGTGAAGAG740.10283776647488814No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGAAGCAGCAGTGAAGAG720.10005836738097224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)