FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357
Total Bases341.6 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA77132.71107339838778No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT1667.042851081883751No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA1195.04879083580823No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT943.9881204921510394No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC893.7759864234196012No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC723.0547305897327113No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA471.9940602460755197Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA471.9940602460755197No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA461.951633432329232No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCTTGGAC381.612218922358931No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT371.5697921086126432No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC351.4849384811200679No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA321.3576580398812048No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT291.230377598642342No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA241.0182435299109036No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT210.8909630886720408No Hit
CAACCAAAAGAGAGACAGCACCATCAAAGAGAGACAGCACCATCAAAGAG210.8909630886720408No Hit
CAACCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAG200.8485362749257531No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGAAC190.8061094611794655No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG170.7212558336868901No Hit
CAACCAAAAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA170.7212558336868901No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG110.46669495120916415No Hit
CAACCAAAAGAGAAGGAGGCCCCATCAAAGAGAGACAGCACCATCAAAGA110.46669495120916415No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG100.42426813746287656Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA100.42426813746287656Clontech Universal Primer Mix Long (96% over 26bp)
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG100.42426813746287656No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG80.3394145099703012No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGA80.3394145099703012No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGTTGGACTGTAGA70.2969876962240136No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACTTGGACTG60.2545608824777259No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGATCAAAGA60.2545608824777259No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGTACCATCAAAGA50.21213406873143828No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGTA50.21213406873143828No Hit
CAACCAAAAGAGAGACAGCACCATCAAAGAGAGACAGCACTTGGACTGTA50.21213406873143828No Hit
GCATCAATCATCACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATG50.21213406873143828No Hit
CAACCAAAAGGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG50.21213406873143828No Hit
CACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG50.21213406873143828No Hit
GCATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAG50.21213406873143828No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC40.1697072549851506Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGACAGCACCGTACTTGGAGTTGGACTGTAGAACCATGTC40.1697072549851506No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAGAG40.1697072549851506No Hit
CAACCAAAAGAGAAGGAGGCACAAGCAGTGGTATCAACGCAGAGTACATG40.1697072549851506Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAG40.1697072549851506No Hit
CAACCAAAAGAGAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGTA40.1697072549851506No Hit
TCCTATCAGTGGCCGCACCGTTACTTGAGTTGGACTGTAGAACCATGTCG40.1697072549851506No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAACACCATCAAAGA30.12728044123886295No Hit
TCCTATCAGTGGCAGCACCGTTACTTGAGTTGGACTGTAGAACCATGTCG30.12728044123886295No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGTTGGACTGTAGAAC30.12728044123886295No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAGCAAAGA30.12728044123886295No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCCATCAAA30.12728044123886295No Hit
CAACAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA30.12728044123886295No Hit
CAACCAAAAGAGAGGCAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA30.12728044123886295No Hit
CAACCAAAAGAGAAGGAGGCATCATCAAAGAGAGACAGCACCATCAAAGA30.12728044123886295No Hit
CAACCAAAAGAGAGACAGCACCCCATCAAAGAGAGACAGCACCCCATCAA30.12728044123886295No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG30.12728044123886295No Hit
GCATCAATCATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTC30.12728044123886295No Hit
CAACCAAAAGAGAAGGAGGCACCACCATCAAAGAGAGACAGCACCACCAT30.12728044123886295No Hit
CAACCAAAAGAGAAGGAGGCACCCTCCATCAAAGAGAGACAGCACCCTCC30.12728044123886295No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC30.12728044123886295Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)