FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21889
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA467821.3714651194664No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT449020.51258623052675No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT401418.33797797980721No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA8193.7416053725615606No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT4462.037553108867468No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCATTCCATC3751.7131892731508978No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA3611.649230206953264No Hit
CAACCAAAAGAGAAGGAGGCACCTAAAAATTGTATACCGCAGATTCAGGC3161.4436474941751565No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCAATCCATC3111.4208049705331445No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC2561.169537210471013No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC2221.0142080497053314No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1500.6852757092603591Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG1430.6532961761615423Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT1280.5847686052355064No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC1190.5436520626798849No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1080.4933985106674585No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA760.34720635935858196No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG660.301521312074558No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT630.2878157978893508No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA580.26497327424733885No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA580.26497327424733885No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA540.24669925533372924No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA520.23756224587692448No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC520.23756224587692448No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA470.21471972223491254No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC410.18730869386449817Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA360.16446617022248616Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT350.1598976654940838No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA340.1553291607656814No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA340.1553291607656814No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA330.150760656037279No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT310.14162364658047422No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG310.14162364658047422No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA290.13248663712366943No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.13248663712366943No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG290.13248663712366943No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA290.13248663712366943No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG290.13248663712366943No Hit
CAACCAAAAGAGAAGGAGGCAGGAAGAATGGAACCAAGTTGGAAAATATA290.13248663712366943No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA270.12334962766686462No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG270.12334962766686462No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT260.11878112293846224No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC260.11878112293846224No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.11421261821005985No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.11421261821005985No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCAGGGCTTCACTTACTCT250.11421261821005985No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.11421261821005985No Hit
CAACCAAAAGAGAAGGAGGCACAACATGAAAGAGAACAACTTGAATGTCA240.10964411348165744No Hit
CAACCAAAAGAGAAGGAGGCACCGAGCCTGGTGCAGTGGTATCAACGCAG240.10964411348165744No Hit
CAACCAAAAGAGAAGGAGGCACAAGCAGTGGTATCAACGCAGAGTACATG240.10964411348165744Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA230.10507560875325506No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT220.10050710402485268No Hit
CAACCAAAAGAGAAGGAGGCCAGGCTCCAAGGAGGTATGGCTGTAGTCAG220.10050710402485268No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)