FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10931
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA448140.99350471137133No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT113910.419906687402799No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA8687.940719055896076No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT4283.915469764888848No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA4143.7873936510840727No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1681.5369133656573049No Hit
CAACCAAAAGAGAAGGAGGCACTCCACTTTTCACACTCAGGAAAACTGGT1531.3996889580093312No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1521.390540664166133No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA890.8141981520446437No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA800.7318635074558595No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT600.5488976305918946No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA570.5214527490622999No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT510.46656298600311047No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC470.4299698106303175No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA430.39337663525752453No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA410.37508004757112795No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA400.36593175372792974No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT400.36593175372792974No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA350.3201902845119385No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA350.3201902845119385No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA330.30189369682554207No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC320.2927454029823438No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA270.2470039337663526Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA240.21955905223675784No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA240.21955905223675784No Hit
GGGCAAAGGGTGAGGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA240.21955905223675784No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA240.21955905223675784No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG210.1921141707071631No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT200.18296587686396487No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA200.18296587686396487No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC190.17381758302076664No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG160.1463727014911719No Hit
TCCTATCAGTGGCCGCAAAGAAGACGAAAAAGTCGCTGGAGTCGATCAAC160.1463727014911719No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG160.1463727014911719No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA160.1463727014911719No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG150.13722440764797364No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA130.11892781996157716No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGCCATGTTGTAGAAC130.11892781996157716No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA130.11892781996157716No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT120.10977952611837892No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGACA120.10977952611837892No Hit
CAACCAAAAGAGAAGGAGGCACTGCTGGACTGAGCTTGTGGTTCCCCCAT120.10977952611837892No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.10977952611837892No Hit
CAACCAAAAGAGAAGGAGGCACACCATCAAAGAGAGACAGCACCCATGTT110.10063123227518067No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.10063123227518067No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA110.10063123227518067No Hit
GGGCAAGGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA110.10063123227518067No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT110.10063123227518067No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.10063123227518067No Hit
CAACCAAAAGAGAAGGAGGCACTGAACCCCAGTGGGGAGACCAGAAGTGC110.10063123227518067No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)