FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17614
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA372721.159305098217327No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC263614.965368456909275No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA14788.391052571817873No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC13267.5281026456228No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA11986.801407970932213No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA8534.84273873055524No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT5693.2303849210855002No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG2231.2660383785625071No Hit
GTGCTGTCTCTCTTTGATGGAATCTTGAGAGATCTGGTTGTTTAAAGTGT2121.2035880549562847No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA1791.0162370841376178No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG1710.9708186669694561No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1260.7153400703985466No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1030.5847621210400817No Hit
GTGCTGTCTCTCTTTGATGGATGTCCTGGGTCCTCCCAATTCTTAGTCCT940.5336664017258999No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA940.5336664017258999No Hit
GTGCTGTCTCTCTTTGATGGAATCAAAAGTTTTCTTTCTTACTAGAGGCT910.5166344952878392No Hit
GTGCTGTCTCTCTTTGATGGACTGACTTCATTCTCAACTCTTAAGAGTGA810.45986147382763715No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA670.38037924378335414No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA660.37470194163733395No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG660.37470194163733395No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA630.3576700351992733No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA600.3406381287612127No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC540.3065743158850914No Hit
GTGCTGTCTCTCTTTGATGGACTTCTAGAACGTCTGGCTGTGCTGTAACT530.3008970137390712No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA480.2725105030089701No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTCACAG480.2725105030089701No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA440.2498012944248893No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG410.23276938798682867No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA400.22709208584080848No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC380.215737481548768No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCAGTCTCTC360.2043828772567276No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA320.18167366867264675No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG310.17599636652662654No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA280.15896446008856593No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA270.1532871579425457No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA270.1532871579425457No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA260.1476098557965255No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA240.13625525150448506No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA240.13625525150448506No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA240.13625525150448506No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA230.13057794935846487No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC230.13057794935846487No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT210.11922334506642444No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG210.11922334506642444No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTGGTTGTCACAGAGAC200.11354604292040424No Hit
GTGCTGTCTCTCTTTGATGGATGGTTCAGGACGTGAGATATTCGGATTCC200.11354604292040424No Hit
GTGCTGTCTCTCTTTGATGGAATGACTTCATTCTCAACTCTTAAGAGTGA200.11354604292040424No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGATGTCTCTC190.107868740774384No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG180.1021914386283638No Hit
GTGCTGTCTCTCTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAGT180.1021914386283638No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA180.1021914386283638No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)