FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5680
Total Bases822.8 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA107218.87323943661972No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA2855.017605633802817No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG2384.190140845070422No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG2263.97887323943662No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC2263.97887323943662No Hit
GTGCTGTCTCTCTTTGATGGAATCTTCAATGCTTTCTTCCTCAAACTGGG2163.8028169014084505No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1773.1161971830985915No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1552.7288732394366195No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA1502.640845070422535No Hit
GTGCTGTCTCTCTTTGATGGAATCGAAAGGGGGGAAAGGCGGGGAAAGGA1362.3943661971830985No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA1212.130281690140845No Hit
GTGCTGTCTCTCTTTGATGGAGGACAAGATTGGAGTGATGTGGCCACAAG1081.9014084507042253No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG771.3556338028169013No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT711.25No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA671.1795774647887325No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT550.9683098591549295Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG430.7570422535211268No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGTAAAGTGAGTCCTCTCAAACCT390.6866197183098592No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA390.6866197183098592No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTCACAGAGACCA370.6514084507042254No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT330.5809859154929577Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT310.5457746478873239No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.528169014084507No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT290.5105633802816901No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA290.5105633802816901No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA270.4753521126760563No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC250.44014084507042256No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG220.3873239436619718No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA200.35211267605633806No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC190.3345070422535211No Hit
GTGCTGTCTCTCTTTGATGGATGGGTGGTGGGTCTTGATCGGGGTCAACA180.31690140845070425No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT180.31690140845070425No Hit
GTGCTGTCTCTCTTTGATGGAATCAAGGCCAAGGAAATGGCTGGGTGGAG180.31690140845070425No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG170.2992957746478873No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA160.28169014084507044No Hit
GTGCTGTCTCTCTTTGATGGAGGGTGGTCGCAGCAAAGGGTTTGGTTTTG150.2640845070422535No Hit
GCAAAAGTTCTCAGGGATGTTAGAGCATAGCAACTCTGCGTTGATACCAC150.2640845070422535No Hit
GTGCTGTCTCTCTTTGATGGAGGTTAGAGCATAGCAACTCTGCGTTGATA150.2640845070422535No Hit
GGTGTTTGGGATGGAATGGTGGAGCGCTTGTTGTAGTGCGCCAGGCGGGA150.2640845070422535No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGAGTACATGGGAAGCAGTGGT140.24647887323943662Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC140.24647887323943662Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGACAAGTCACCCAAGCAGGTTGACATCAGGT130.22887323943661972No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTCACAG120.21126760563380279No Hit
CTCCAAGTAACGGTGCTGTCGATGAGCACGATGCAATTCTTCACCAGGGT120.21126760563380279No Hit
GTGCTGTCTCTCTTTGATGGAAGACAAGATTGGAGTGATGTGGCCACAAG120.21126760563380279No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC120.21126760563380279No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT110.1936619718309859No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC110.1936619718309859No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT110.1936619718309859No Hit
GTGCTGTCTCTCTTTGATGGATATCAAGGCCAAGGAAATGGCTGGGTGGA110.1936619718309859No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGGGTGATCTAAAACACTCTTTACGC110.1936619718309859No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC110.1936619718309859No Hit
GTGCTGTCTCTCTTTGATGGAATCTGTGTATGTGGGTGTGTGTTTTAAGC110.1936619718309859No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA110.1936619718309859No Hit
GTGCTGTCTCTCTTTGATGGAATATTCAATGCTTTCTTCCTCAAACTGGG100.17605633802816903No Hit
GTGCTGTCTCTCTTTGATGGACAAGTCACCCAAGCAGGTTCACATCAGGT100.17605633802816903No Hit
GTGCTGTCTCTCTTTGATGGAGGTTAGAGCATAGCAACTCTGGTGCCTCC100.17605633802816903No Hit
GTGCTGTCTCTCTTTGATGGATGTGTGTGCCTCCTTCTCTTTTGGTTGTC100.17605633802816903No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCCT90.15845070422535212No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGGTATCAACGCAGAGTACATGGG90.15845070422535212Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGGAATGGTGCGGCCACTGATAGGATCACAGAGACCAAG80.14084507042253522No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA80.14084507042253522No Hit
GCAAAAGTTCTCAGGGATGTCAGAGCATAGCAACTCTGCGTTGATACCAC80.14084507042253522No Hit
GTGCTGTCTCTCTTTGATGGATGCCTCCTTCTCTTTTGGTTGTCACAGAG70.12323943661971831No Hit
CTCCAAGTAACGGTGCTTTCTCTTCACCCTTTGCCCTCACAGAGACCAAG70.12323943661971831No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA70.12323943661971831Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGC70.12323943661971831No Hit
CTCCAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTGC60.10563380281690139No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA60.10563380281690139No Hit
GTGCTGTCTCTCTTTGATGGAACTTTAGCAGCAGCAGCAGCAGCAGCAGC60.10563380281690139No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGGAGTGTATCAGTCAGCTCC60.10563380281690139No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTTAGTGAGGACC60.10563380281690139No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA60.10563380281690139No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT60.10563380281690139No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG60.10563380281690139No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)