FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5680
Total Bases822 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA105818.62676056338028No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA2865.035211267605634No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT2845.0No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA2434.278169014084507No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC2294.031690140845071No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2273.996478873239437Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC2193.855633802816901No Hit
CAACCAAAAGAGAAGGAGGCACCGTCAGGGCAGTGGAAGCGTAGAGTGGG2133.75No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT1512.658450704225352No Hit
GCATCAATCATCACAGGGTTGGGGGTAGGGTCACCGATCAACAGGATCAC1332.341549295774648No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT1192.095070422535211No Hit
GCATCAATCATCACAGGGTTGGTTCCTTCTAGAGGCTCTGCAGGAGATTC1182.0774647887323945No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1061.8661971830985915Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC691.2147887323943662No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT661.1619718309859155No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA480.8450704225352111Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC410.721830985915493No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA400.7042253521126761No Hit
GCATCAATCATCACAGGGTTGGGTAGGAAGGAACTTGGAACAGAACAGAA380.6690140845070423No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA370.6514084507042254No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT310.5457746478873239No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC280.49295774647887325Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC270.4753521126760563No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA250.44014084507042256No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA240.42253521126760557Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA230.4049295774647887No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA200.35211267605633806No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT200.35211267605633806No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA200.35211267605633806No Hit
GCATCAATCATCACAGGGTTGGGAACAGCTCGTACACTTGCCATTCTCTG180.31690140845070425No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC180.31690140845070425Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACGACACCCATGTTGTCATGGCTTAACATC180.31690140845070425No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG170.2992957746478873No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA170.2992957746478873No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT160.28169014084507044No Hit
TCCTATCAGTGGCCGCAAAGAGAGCTACTCCATCTACGTGTACAAGGTGC140.24647887323943662No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA130.22887323943661972No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG130.22887323943661972Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA130.22887323943661972No Hit
GCATCAATCATCACAGGGTTGGCCGACCTGATGTCAACCTGCTTGGGTGA120.21126760563380279No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCATGT120.21126760563380279No Hit
CAACCAAAAGAGAAGGAGGCACAGAATAATCAAGAATGAAGTTAAGAAGC120.21126760563380279No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG120.21126760563380279Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCAGAAGAAAAGAGGAAGAAGAAGAAGAGGAG110.1936619718309859No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA110.1936619718309859No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA100.17605633802816903No Hit
GCATCAATCATCACAGGGTTGGTCGCTGTAAATGCAATTAGGCCAGCTGT100.17605633802816903No Hit
CAACCAAAAGAGAAGGAGGCACACACATCCATCAAAGAGAGACAGCACCC100.17605633802816903No Hit
CAACCAAAAGAGAAGGAGGCACCAGAGTTGCTATGCTCTAACCTCCATCA100.17605633802816903No Hit
CAACCAAAAGAGAAGGAGGAAGAAGAAAAGAGGAAGAAGAAGAAGAGGAG90.15845070422535212No Hit
GCATCAATCATCACAGGGTTGGCCGACCTGATGTGAACCTGCTTGGGTGA90.15845070422535212No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA80.14084507042253522No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA80.14084507042253522No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA80.14084507042253522No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT70.12323943661971831No Hit
CAACCAAAAGAGAAGGAGGCACTTGCTTTGTGGATGATCAACTCAACCCT70.12323943661971831No Hit
CAACCAAAAGAGAAGGAGGCATCCATCAAAGAGAGACAGCACCCATGTTG70.12323943661971831No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGCCATGTTGTAGAAC70.12323943661971831No Hit
GGGCAAAGGGTGAAGAGAAAGAATACCCATGTACTCTGCGTTGATACCAC70.12323943661971831Clontech SMART CDS Primer II A (95% over 21bp)
TCCTATCAGTGGCCGCACCATTCCATCCCAAACACCCCATGTTGTAGAAC70.12323943661971831No Hit
TCCTATCAGTGGCCGCAAAACCGGGGGCAAGAGAAAGCCCTACCACAAGA60.10563380281690139No Hit
TCCTATCAGTGGCAGCACCGTTACTGGAGCCATGTTGTAGAACCATGTCG60.10563380281690139No Hit
TCCTATCAGTGGCCGCATTCAGAGGCTGGCCCCCCTCCTTGCCTTTACTC60.10563380281690139No Hit
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG60.10563380281690139Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAGGAGGCACCGTCAGGGCAGTGGAAGCGTAGAGTGGGC60.10563380281690139No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)