FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27031
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1368650.630757278680036No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA415615.374939883837078No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA13865.127446265398986No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA9523.521882283304354No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT6862.5378269394399022No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT3731.3798971551181978No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC2620.9692575191446857No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC2570.950760238244978No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT1270.46983093485257665No Hit
GCATCAATCATCACAGGGTTGGGTAGGAAGGAACTTGGAACAGAACAGAA1130.418038548333395No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1080.39954126743368723No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1000.3699456179941549No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT970.3588472494543302No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT910.3366505123746809No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC780.2885575820354408No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT770.28485812585549924No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT760.2811586696755577No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA750.27745921349561614No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA520.19237172135696054Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCATGT520.19237172135696054No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA520.19237172135696054No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC520.19237172135696054No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT500.18497280899707744No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT490.18127335281713589No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT490.18127335281713589No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA490.18127335281713589No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA490.18127335281713589No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT450.1664755280973697No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT430.1590766157374866No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA400.14797824719766195No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT400.14797824719766195No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT400.14797824719766195No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT370.1368798786578373No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA350.1294809662979542No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT330.12208205393807109No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT330.12208205393807109No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA310.11468314157818801No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT300.11098368539824645No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA290.10728422921830491No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)