FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7059
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA334147.32965009208103No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA2323.286584502054115No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC2303.2582518770364075No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC2153.045757189403598No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA2123.0032582518770368No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG1682.379940501487463No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG1572.2241110638900694No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT951.345799688341125No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA670.9491429380932143No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA620.8783113755489446No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTATAGCAACTCTGC580.8216461255135288No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA570.8074798130046749No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA550.7791471879869669No Hit
GTGCTGTCTCTCTTTGATGGAATCCATGCCTGAATCTGCGGTATACAATT480.6799830004249894No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT460.6516503754072814Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA460.6516503754072814No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA460.6516503754072814No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT430.6091514378807197Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG420.5949851253718658No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA320.45332200028332625No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG300.4249893752656183No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTTATAGCAACTC280.39665675024791053No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTCACAGAGACCA230.3258251877036407No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC220.3116588751947868Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT200.2833262501770789No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA200.2833262501770789No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT190.26915993766822494No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC180.254993625159371No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.24082731265051707No Hit
GGTGTTTGGGATGGAATGGTGGAGCGCTTGTTGTAGTGCGCCAGGCGGGA170.24082731265051707No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT160.22666100014166313No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCTTATAGCAACTCTG150.21249468763280915No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.21249468763280915No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCGGTCATCCCCAAGC140.19832837512395526No Hit
CTCCAAGTAACGGTGCTGTCTGCTGACTGGTGCAGGGCTCCTGGTGCTGA130.1841620626151013No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT130.1841620626151013No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.16999575010624735No Hit
GCAAAAGTTCTCAGGGATGGGTCATCCCCAAGCAGCTTATAGCAACTCTG120.16999575010624735No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCCCCTGGTGCTGA120.16999575010624735No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.16999575010624735No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC110.1558294375973934No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA110.1558294375973934No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGGTATCAACGCAGAGTACATGGG110.1558294375973934Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAGCAGCTTCACCACTTCATCATAGATGACA100.14166312508853945No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC100.14166312508853945No Hit
GTGCTGTCTCTCTTTGATGGAGACCTAAGTAGGGCTAAAATTAGCAGCTG90.1274968125796855No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA90.1274968125796855Clontech SMART CDS Primer II A (96% over 26bp)
CTCCAAGTAACGGTGCTGTCTGCTGGCTGATGCAGGGCTCCTGGTGCTGA90.1274968125796855No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG90.1274968125796855No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG80.11333050007083156No Hit
CTCCAAGTAACGGTGCTGTCTACTGGCTGGTGCAGGGCTCCTGGTGCTGA80.11333050007083156No Hit
GTGCTGTCTCTCTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTATG80.11333050007083156No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGAGTACATGGGAAGCAGTGGT80.11333050007083156Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA80.11333050007083156No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC80.11333050007083156No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGACTCCTGGTGCTGA80.11333050007083156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)