FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7059
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length137-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA324745.99801671624876No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA3885.496529253435331No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2353.3290834395806774Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT2303.2582518770364075No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT2072.932426689332767No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1652.337441563960901No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1522.1532795013457995No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1391.9691174387306984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA680.9633092506020683No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT630.8924776880577986No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA600.8499787505312366No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA590.8358124380223828Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT530.7508145629692592No Hit
CAACCAAAAGAGAAGGAGGCACCTAAAAATTGTATACCGCAGATTCAGGC490.6941493129338433No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC480.6799830004249894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA420.5949851253718658No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC380.5383198753364499No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA370.5241535628275961No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA340.48165462530103414No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC290.4108230627567644No Hit
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG270.3824904377390565Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA230.3258251877036407No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA230.3258251877036407Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG220.3116588751947868Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA210.2974925626859329No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA200.2833262501770789No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT200.2833262501770789No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA180.254993625159371No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG170.24082731265051707Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGAGAGCTACTCCATCTACGTGTACAAGGTGC170.24082731265051707No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA170.24082731265051707No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA170.24082731265051707No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA160.22666100014166313No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA140.19832837512395526No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA120.16999575010624735No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCG110.1558294375973934No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT110.1558294375973934No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC110.1558294375973934Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGGGCTCAAGGCATTCTACAAGGGCACTGTCCC110.1558294375973934No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA110.1558294375973934No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG100.14166312508853945No Hit
GGGCAAAGGGTGAAGAGAAAGGGGGCAAATGAACAAAATGAAAGGAGGCA100.14166312508853945No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA100.14166312508853945No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA100.14166312508853945No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGACA90.1274968125796855No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA90.1274968125796855No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.1274968125796855No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAGAGGAGGCA90.1274968125796855No Hit
GCATCAATCATCACAGGGTACATGGGAGCAGTGGTATCAACGCAGAGTAC80.11333050007083156Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGGAAGGAGGCA80.11333050007083156No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAGCAAAATGAAAGGAGGCA80.11333050007083156No Hit
CAACCAAAAGAGAAGGAGGCAGCTGCTAATTTTAGCCCTACTTAGGTCTC80.11333050007083156No Hit
CAACCAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTAG80.11333050007083156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)