FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20633
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1141255.30945572626376No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA18028.733582125720933No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA15937.720641690495808No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT7173.4750157514660978No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC2371.1486453739155722No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC2291.1098725342897302No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT1680.8142296321426841No Hit
CAACCAAAAGAGAAGGAGGGAAGAAGGGAAGGAAGGAAGGGAGGAAAAGT1300.630058643919934No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT870.4216546309310328No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC840.40711481607134203No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC710.34410895167934863No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT700.3392623467261184No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT610.29564290214704597No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA550.2665632724276644No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT510.24717685261474337No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT500.2423302476615131No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT460.22294382784859207No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT450.2180972228953618No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT440.21325061794213152No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA430.2084040129889013No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT430.2084040129889013No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA420.20355740803567102No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA410.19871080308244077No Hit
CAACCAAAAGAGAAGGAGGGAAGGAGGGAAGAAGGGAAGGAAGGAAGGGA380.18417098822274997No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT370.1793243832695197No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG350.1696311733630592No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA350.1696311733630592No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA330.15993796345659866No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC330.15993796345659866No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT300.14539814859690786No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.14539814859690786No Hit
CAACCAAAAGAGAAGGAGGCGTTGAAAGACTATTTTAGGAAATAATAGCT290.1405515436436776No Hit
CAACCAAAAGAGAAGGAGGGATGGAGAGAAGGAAGGAAGGAGGGAAGAAG280.13570493869044734No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA280.13570493869044734No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT260.1260117287839868No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA260.1260117287839868No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT260.1260117287839868No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT260.1260117287839868No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT260.1260117287839868No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA260.1260117287839868No Hit
GCATCAATCATCACAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGT240.1163185188775263No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT240.1163185188775263No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT230.11147191392429603No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.10662530897106576No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA220.10662530897106576No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA220.10662530897106576No Hit
CCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT210.10177870401783551No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)