FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3406
Total Bases493.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA169749.823840281855546No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG1394.081033470346448No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG1073.141514973576042No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC822.4075161479741634No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA581.7028772753963595No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA561.6441573693482088No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA561.6441573693482088No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA391.1450381679389312No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT361.0569583088667058Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA330.9688784497944805No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC260.7633587786259541No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG250.7339988256018791No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA240.7046388725778039No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA230.6752789195537288No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT220.6459189665296535No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA190.5578391074574282No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT190.5578391074574282No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA180.5284791544333529No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT170.49911920140927774Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA170.49911920140927774No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG170.49911920140927774No Hit
GTGCTGTCTCTCTTTGATGGAGGTAGACATGGGAGACTTTTCATTTTGTT160.46975924838520255No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTCACAGAGACCA150.4403992953611274No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA140.4110393423370522No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT130.38167938931297707No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA130.38167938931297707No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGAGTACATGGGAAGCAGTGGT120.35231943628890194Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.35231943628890194No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGGTATCAACGCAGAGTACATGGG100.2935995302407516Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA100.2935995302407516No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC90.26423957721667646No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA90.26423957721667646No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC90.26423957721667646No Hit
GTGCTGTCTCTCTTTGATGGACAAGTCACCCAAGCAGGTTGACATCAGGT80.23487962419260128No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA70.2055196711685261No Hit
CTCCAAGTAACGGTGCTGAGAAAGCAGAAATGAGTTCAGCAAAGTGAGGA70.2055196711685261No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC70.2055196711685261No Hit
GTGCTGTCTCTCTTTGATGGAGGGTGGTCGCAGCAAAGGGTTTGGTTTTG60.17615971814445097No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG60.17615971814445097No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT60.17615971814445097No Hit
CTCCAAGTAACGGGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA60.17615971814445097No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCCGGTGCTGA50.1467997651203758No Hit
CTCCAAGTAACGGTGCTGTCTACTGGCTGGTGCAGGGCTCCTGGTGCTGA50.1467997651203758No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.1467997651203758No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC50.1467997651203758Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTCCGCTGGCTGGTGCAGGGCTCCTGGTGCTGA50.1467997651203758No Hit
GCAAAAGTTCTCAGGGATGTTAGCAACTCTGCGTTGATACCACTGCTTCC40.11743981209630064Clontech Universal Primer Mix Long (96% over 25bp)
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGGAAGCAGTGGTATCAACGC40.11743981209630064No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGTTGGTGCAGGGCTCCTGGTGCTGA40.11743981209630064No Hit
CTCCAAGTAACGGCGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA40.11743981209630064No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGTTGA40.11743981209630064No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAA40.11743981209630064No Hit
CTCCAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTGC40.11743981209630064No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTACTGA40.11743981209630064No Hit
GGTGTTTGGGATGGAATGGTGCGGCCACTGATAGGATCACAGAGACCAAG40.11743981209630064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)