FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3406
Total Bases493.4 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA169449.73576042278333No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1363.992953611274222No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1353.9635936582501468Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1103.2295948326482677No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT591.7322372284204346No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT561.6441573693482088No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA521.5267175572519083No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT441.291837933059307No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC351.0275983558426305Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA320.9395184967704051No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA320.9395184967704051Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA300.8807985907222547No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA280.8220786846741044No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT240.7046388725778039No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC240.7046388725778039No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT220.6459189665296535No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.5578391074574282No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA180.5284791544333529No Hit
GCATCAATCATCACAGGGTTGGGGGTAAGGTCACTGATCAACAGGATCCC180.5284791544333529No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC170.49911920140927774No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT170.49911920140927774No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA150.4403992953611274No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC140.4110393423370522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA130.38167938931297707No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA110.32295948326482676No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA110.32295948326482676No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA90.26423957721667646No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA80.23487962419260128No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCG80.23487962419260128No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC70.2055196711685261Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA70.2055196711685261Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA70.2055196711685261No Hit
GCATCAATCATCACAGGGTTGGGAACAGCTCGTACACTTGCCATTCTCTG60.17615971814445097No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG50.1467997651203758No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA50.1467997651203758No Hit
CAACCAAAAGAGAAGGAGGCACAGAGTGAGGCCACAGTGCATCATCCGAT50.1467997651203758No Hit
TCCTATCAGTGGCCGCAAAGAAGACGAAAAAGTCGCTGGAGTCGATCAAC50.1467997651203758No Hit
GGGCAAAGGGTGAAAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA50.1467997651203758No Hit
GGGCAAAGGGTGAAGAGAAAGAATACCCATGTACTCTGCGTTGATACCAC50.1467997651203758Clontech SMART CDS Primer II A (95% over 21bp)
GCATCAATCATCACAGGGTTGGCCGACCTGATGTCAACCTGCTTGGGTGA40.11743981209630064No Hit
GGGAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAAG40.11743981209630064No Hit
GCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAAG40.11743981209630064No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40.11743981209630064Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTGAAGAGAAAGAAGGCAAATGAACAAAATGAAAGGAGGCA40.11743981209630064No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA40.11743981209630064No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGTAAATGAACAAAATGAAAGGAGGCA40.11743981209630064No Hit
TCCTATCAGTGGCCGCACCGTTACTGGAGCCATGTTGTAGAACCATGTCG40.11743981209630064No Hit
TCCTATCAGTGGCCGCAAAGTACATGGGAAGCAGTGGTATCAACGCAGAG40.11743981209630064Clontech Universal Primer Mix Long (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)