FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8257
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA379946.009446530216785No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC8079.77352549352065No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA5686.879011747608089No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT3133.7907230228896696No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG1541.865084171006419No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG1061.2837592345888338No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC690.8356545961002786No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA670.8114327237495458No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA660.7993217875741796No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA580.7024342981712487No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA490.5934358725929514No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA430.5207702555407533No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA420.5086593193653869No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA380.4602155746639215No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG370.4481046384885552No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA340.4117718299624561No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG320.3875499576117234No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC320.3875499576117234No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA320.3875499576117234No Hit
GTGCTGTCTCTCTTTGATGGACCGAGGAGCAGAGTCGGTGACTAACCAGT310.375439021436357No Hit
CTCCAAGTAACGGTGCGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG310.375439021436357No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTCACAG300.3633280852609907No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT260.31488434055952524No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCAGTCTCTC260.31488434055952524No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT260.31488434055952524No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA260.31488434055952524No Hit
GCAAAAGTTCTCAGGGATGTCCTGCAGAAATGGATGATTTTCATTTCCTG230.2785515320334262No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT220.26644059585805985Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTCACAGAGACCA190.23010778733196074No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG180.21799685115659442No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.20588591498122805No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA160.1937749788058617No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC160.1937749788058617No Hit
GTGCTGTCTCTCTTTGATGGAGCCTCCTGGCTCCCACCGTCCACTCATCA150.18166404263049535No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA140.16955310645512897No Hit
CTCCAAGTAACGGTGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG140.16955310645512897No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG130.15744217027976262No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG130.15744217027976262No Hit
CTCCAAGTAACGGGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.14533123410439627No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA110.13322029792902992No Hit
GTGCTGTCTCTCTTTGATGGACATTTTGACCCCTTCAGAATGGACATGAG110.13322029792902992No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA110.13322029792902992No Hit
CTCCAAGTAACGGTGCTGTCTGCTGACTGGTGCAGGGCTCCTGGTGCTGA100.12110936175366356No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA100.12110936175366356No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGGCTCTC90.10899842557829721No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTGGTTGTCACAGAGAC90.10899842557829721No Hit
GTGCTGTCTCTCTTTGATGGAATCAAAATATCCACGGCCATGATCTTCTC90.10899842557829721No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA90.10899842557829721No Hit
GTGCTGTCTCTCTTTGATGGAATGCCGCAGATGTTTTTAAAGCACCTTGG90.10899842557829721No Hit
GTGCTGTCTCTCTTTGATGGTGCCCCCTTCTCTTTTGGTTGTCACAGAGA90.10899842557829721No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGACTCCTGGTGCTGA90.10899842557829721No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC90.10899842557829721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)