FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8257
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA369344.72568729562795No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA126815.356667070364537No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT5686.879011747608089No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1481.7924185539542208No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1071.2958701707642No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA800.9688748940293085Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA640.7750999152234468No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA630.7629889790480804No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA630.7629889790480804No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT610.7387671066973477No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT440.5328811917161197No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA440.5328811917161197No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT420.5086593193653869No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA390.4723265108392879No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA360.43599370231318885No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC360.43599370231318885No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA340.4117718299624561No Hit
CAACCAAAAGAGAAGGAGGCACCACCATGGAAACAGGAGAGGTGAGGAGA340.4117718299624561No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC320.3875499576117234No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA290.35121714908562435No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT290.35121714908562435No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA280.33910621291025794Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA270.3269952767348916No Hit
CAACCAAAAGAGAAGGAGGCACATGTATCACACCTGTTCAGTGGCAGCTT260.31488434055952524No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT240.29066246820879255No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC240.29066246820879255Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA230.2785515320334262No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA190.23010778733196074No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA180.21799685115659442No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCATCAAAGA180.21799685115659442No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA170.20588591498122805No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA170.20588591498122805No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA150.18166404263049535No Hit
CAACCAAAAGAGAAGGAGGCACCCGACAGTGTGGCCACCTCTGGCTCCGT150.18166404263049535No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA140.16955310645512897No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG130.15744217027976262No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAACAAAGA130.15744217027976262No Hit
GGGCAAAGGGTGAAAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA130.15744217027976262No Hit
GCATCAATCATCACAGGGTTGGACGGGCTGTCTGAGCAAGGATCTCTGTC130.15744217027976262No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA120.14533123410439627No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA110.13322029792902992No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAGCAAAATGAAAGGAGGCA110.13322029792902992No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT110.13322029792902992No Hit
CAACCAAAAGAGAAGGAGGCACTGGGACACACGTGGGGGCTGCCTGGGTT110.13322029792902992No Hit
GGGCAAAGGGTGAAGAGAAAGGGGGCAAATGAACAAAATGAAAGGAGGCA100.12110936175366356No Hit
GGGCAAAGGGTGAGGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA100.12110936175366356No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG100.12110936175366356Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTAAAGGGCAAATGAACAAAATGAAAGGAGGCAAGGGCTGC90.10899842557829721No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCCATGTTGT90.10899842557829721No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.10899842557829721No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT90.10899842557829721No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.10899842557829721No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.10899842557829721No Hit
CACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.10899842557829721No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGGACAAAATGAAAGGAGGCA90.10899842557829721No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA90.10899842557829721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)