FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14757
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA626142.427322626550115No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA168911.445415734905469No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC8325.6380023039913265No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA7595.143321813376703No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT3552.4056380023039914No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG2311.5653588127668225No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG2311.5653588127668225No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA1771.1994307786135394No Hit
GTGCTGTCTCTCTTTGATGGAATCAATAACCTTCGAGATAGCTCCAATTC1531.0367960967676357No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1020.6911973978450905No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT800.5421156061530121No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG780.5285627159991868No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG720.48790404553771094No Hit
GTGCTGTCTCTCTTTGATGGAATCTCATGTCAAATTATAATTCCCAGTAT720.48790404553771094No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG690.467574710306973No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG650.44046892999932236No Hit
GTGCTGTCTCTCTTTGATGGAATCCTTTCCCGTATTAAACAAACCAGAGA640.4336924849224097No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA570.38625736938402117No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG460.311716473537982No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAA420.2846106932303314No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT370.2507284678457681No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA370.2507284678457681No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCAGTCTCTC350.23717557769194283No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG330.2236226875381175No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA310.2100697973842922No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTCACAG280.18974046215355425No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC260.17618757199972895No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC250.1694111269228163No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA240.16263468184590363No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA230.155858236768991No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA230.155858236768991No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT220.14908179169207833No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT210.1423053466151657Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.1423053466151657No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT210.1423053466151657Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.1423053466151657No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC210.1423053466151657No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAA200.13552890153825303No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTCACAGAGACCA200.13552890153825303No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG200.13552890153825303No Hit
GTGCTGTCTCTCTTTGATGGAATCCTCGTGAGATCATGTTGTTTAAAAGT190.12875245646134037No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA190.12875245646134037No Hit
GTGCTGTCTCTCTTTGATGGAGTATAACATTATTCAGAGTCTCCACAGCA190.12875245646134037No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTGGTTGTCACAGAGAC180.12197601138442773No Hit
GTGCTGTCTCTCTTTGATGGAATCTAACATTATTCAGAGTCTCCACAGCA180.12197601138442773No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.11519956630751509No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA170.11519956630751509No Hit
GTGCTGTCTCTCTTTGATGGATGTCCTGGGTCCTCCCAATTCTTAGTCCT160.10842312123060242No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA160.10842312123060242No Hit
GTGCTGTCTCTCTTTGATGGAATATAACATTATTCAGAGTCTCCACAGCA160.10842312123060242No Hit
GTGCTGTCTCTCTTTGATGGATTTTTTAATCAAGTTGTTTTTATTGTTGA150.10164667615368977No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC150.10164667615368977No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG150.10164667615368977No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA150.10164667615368977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)