FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10883
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length138-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT732867.33437471285491No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2562.3522925663879444Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC2202.0215014242396396No Hit
GCATCAATCATCACAGGGTTGGGGGCAAGGTCACAGATCAACAGGATCCC2131.9571809243774694No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1661.5253147110171827No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT1181.084259854819443No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1050.9648074979325554No Hit
CAACCAAAAGAGAAGGAGGCACCTAAAAATTGTATACCGCAGATTCAGGC930.8545437838831206No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT640.5880731415969861No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT610.5605072130846274No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT530.4869980703850041No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT510.4686207847100983No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC470.4318662133602867Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA440.40430028484792796Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTTGGGGGTAAGGTCACAGATCAACAGGATCCC430.39511164201047505No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC390.3583570706606634No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT370.33997978498575765No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT360.3307911421483047No Hit
CAACCAAAAGAGAAGGAGGCACCAGAGCCCATCTTCAGAGCACTCTGGGA300.2756592851235873No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT280.2572819994486814No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA270.24809335661122853No Hit
CAACCAAAAGAGAAGGAGGCACAAGAACAAGAAGATTGGAAGAAATATAT250.22971607093632268No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.22971607093632268No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.22052742809886983No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.22052742809886983No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT230.2113387852614169No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT230.2113387852614169No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT230.2113387852614169No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.20215014242396398No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT210.19296149958651107No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT210.19296149958651107No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC200.18377285674905816Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT190.17458421391160525No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT190.17458421391160525No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG180.16539557107415234No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC180.16539557107415234Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT170.15620692823669946No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCC150.13782964256179364No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT140.1286409997243407No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT130.11945235688688781No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA130.11945235688688781Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT130.11945235688688781No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA120.11026371404943491No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA120.11026371404943491No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT120.11026371404943491No Hit
CCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT120.11026371404943491No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC120.11026371404943491No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA110.10107507121198199No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT110.10107507121198199No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)