FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14201
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT819057.67199492993451No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA299821.111189352862475No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA6574.626434758115626No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1380.9717625519329625No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC900.6337581860432363No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG730.5140483064572918No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT590.41546369973945496No Hit
CAACCAAAAGAGAAGGGGGCACCACAGGTTTCTGGATCCAGCCCCTGACA440.3098373353989156No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT420.2957538201535103No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT400.2816703049081051No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT370.2605450320399972No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT370.2605450320399972No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT310.21829448630378145No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.2112527286810788No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG300.2112527286810788No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT280.19716921343567356No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC280.19716921343567356No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT270.1901274558129709No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT270.1901274558129709No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT270.1901274558129709No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG250.17604394056756567No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT240.16900218294486302No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.1619604253221604No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.1619604253221604No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.1549186676994578No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.1549186676994578No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG190.13379339483134992No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT180.12675163720864727No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC180.12675163720864727No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA170.11970987958594465No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT170.11970987958594465No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA160.11266812196324204No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT160.11266812196324204No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCTCTGGGCTTCACTTACTCT160.11266812196324204No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT150.1056263643405394No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG150.1056263643405394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)