FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10746
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA343731.98399404429555No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT177816.545691420063278No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA172416.04317885724921No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT2051.9076865810534152No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT1511.4051740182393448No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1281.191140889633352No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1231.1446119486320492No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1121.0422482784291829No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA880.8189093616229295No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG680.6327935976177183No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA580.5397357156151126Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT500.4652894100130281No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA420.3908431044109436No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA420.3908431044109436No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA390.3629257398101619No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT380.35361995160990134No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG380.35361995160990134No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA370.3443141634096408No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC360.33500837520938026No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCATCAAAGA350.3257025870091197No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG310.2884794342080774No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA310.2884794342080774No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG290.2698678578075563No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA290.2698678578075563No Hit
CAACCAAAAGAGAAGGAGGCACCTGGCCCCAGAGGAAGGGATATAAGCAG280.2605620696072957No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA280.2605620696072957No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA270.25125628140703515No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT270.25125628140703515No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA260.24195049320677461No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC250.23264470500651405No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA240.2233389168062535No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA240.2233389168062535Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA230.21403312860599294No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA210.1954215522054718No Hit
CACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG200.18611576400521124No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.17680997580495067No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC190.17680997580495067Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT180.16750418760469013No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA180.16750418760469013No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAACAAAGA170.15819839940442956No Hit
GCATCAATCATCAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTC160.148892611204169No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA160.148892611204169No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGGCAGCACCATCAAAGA160.148892611204169No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA160.148892611204169No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACGGCACCATCAAAGA150.13958682300390843No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA150.13958682300390843No Hit
CAACCAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTAG150.13958682300390843No Hit
CAACCAAAAGAGAAGGAGGCACAGCCAGAAGGAGCCCAAGGATTCTCTGG150.13958682300390843No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA150.13958682300390843No Hit
CAACCAAAAGAGAAGGAGGCCCCATCAAAGAGAGACAGCACCATCAAAGA140.13028103480364786No Hit
GCATCAATCATCACAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGT130.12097524660338731No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGTACCATCAAAGA120.11166945840312675No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA120.11166945840312675No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA120.11166945840312675No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG120.11166945840312675Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCCATGTTGT120.11166945840312675No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG120.11166945840312675No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC110.10236367020286619Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACAAGCAGTGGTATCAACGCAGAGTACATG110.10236367020286619Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAGACAGCACCATCAAAGAGAGACAGCACCATCAAAGAG110.10236367020286619No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG110.10236367020286619Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)