FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27912
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1322847.39180280882774No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT847930.377615362568072No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC2760.9888220120378332No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA2060.7380338205789624No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1970.7057896245342505No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT1880.6735454284895385No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1490.5338205789624535No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1470.5266552020636285No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1310.4693321868730295No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1310.4693321868730295No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG1300.46574949842361707No Hit
GTGCTGTCTCTCTTTGATGGAATCATTCATGTTTTTATCTCCATAACTTT1000.35826884494124395No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG990.35468615649183144No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG850.30452851820005733No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT820.29378045285182003No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT730.26153625680710807No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA580.20779593006592148No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGATCCAGGAGTGGGGCCCATT570.204213241616509No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG560.20063055316709658No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA490.17555173402120952No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG480.17196904557179707No Hit
GTGCTGTCTCTCTTTGATGGAGGTCTTCCTTGTCGTTCTCCACCTCCGGC470.16838635712238462No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT470.16838635712238462Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT430.1540556033247349No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT420.15047291487532244No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG410.14689022642591001No Hit
GGTGTTTGGGATGGAATGGTGTTCCACGCAGGTGGTTCGCAGAAGCAGCA380.13614216107767269No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT370.13255947262826023Clontech Universal Primer Mix Long (96% over 26bp)
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG360.1289767841788478No Hit
GTGCTGTCTCTCTTTGATGGAAAGAAGTAGACATGGGAGACTTCATTTTG350.12539409572943536No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG340.12181140728002293No Hit
GTGCTGTCTCTCTTTGATGGAATCCTCCTCCACGCGGAACTGCAGATCCT340.12181140728002293No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG330.11822871883061048No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA330.11822871883061048No Hit
GTGCTGTCTCTCTTTGTGGAATCGCTACAGATCCAGGAGTGGGGCCCATT330.11822871883061048No Hit
GTGCTGTCTCCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT300.10748065348237318No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA290.10389796503296074No Hit
GGTGTTTGGGATGGAATGGTGACCCACGCAGGTGGTTCGCAGAAGCAGCA280.10031527658354829No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA280.10031527658354829No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.10031527658354829No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)