FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17217
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT838048.67282337224836No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT240213.951327176627752No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA15659.089853052215833No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT7764.507173142823953No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1500.8712319219376198No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1390.8073415809955277Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1350.7841087297438577No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT930.5401637916013242No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT670.3891502584654702No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC650.3775338328396352Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA540.31364349189754315Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT520.3020270662717082No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT500.29041064064587324No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT410.23813672532961605No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT360.20909566126502874No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG350.2032874484521113No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT350.2032874484521113No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT330.19167102282627635No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA320.1858628100133589No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC320.1858628100133589No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT310.18005459720044142No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.17424638438752396No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.16843817157460647No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.16843817157460647No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT280.16262995876168904No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG270.15682174594877157No Hit
CAACCAAAAGAGAAGGAGGCACCGAGCCTGGTGCAGTGGTATCAACGCAG260.1510135331358541No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG260.1510135331358541No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG250.14520532032293662No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.14520532032293662No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC240.13939710751001916Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT240.13939710751001916No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA240.13939710751001916No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT240.13939710751001916No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA230.1335888946971017No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA220.12778068188418423No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA210.12197246907126677No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCATCAAAGA210.12197246907126677No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT200.11616425625834931No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT190.11035604344543185No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA190.11035604344543185Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT190.11035604344543185No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT180.10454783063251437No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG180.10454783063251437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)