FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14860
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA767351.63526244952894No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA174011.709286675639301No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC7595.107671601615074No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA5333.5868102288021535No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT3002.0188425302826376No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG1951.3122476446837146No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG1380.9286675639300135No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1230.8277254374158817No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG1070.7200538358008076No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1030.693135935397039No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG880.5921938088829072No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG850.5720053835800807No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA690.46433378196500674No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG530.35666218034993274No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT530.35666218034993274No Hit
GTGCTGTCTCTCTTTGATGGATGTCCTGGGTCCTCCCAATTCTTAGTCCT490.32974427994616423No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA460.3095558546433378No Hit
GTGCTGTCTCTCTTTGATGGTCGCAGCAAAGGGTTTGGTTTTGTATGTTT380.2557200538358008No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG340.2288021534320323No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA320.21534320323014802No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG310.20861372812920592No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT310.20861372812920592Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.2018842530282638No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG270.1816958277254374No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCAGTCTCTC260.17496635262449528No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA260.17496635262449528No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA260.17496635262449528No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC250.16823687752355315No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT240.16150740242261105Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA240.16150740242261105No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG230.1547779273216689No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.1480484522207268No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.13458950201884254No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC200.13458950201884254No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA190.1278600269179004No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG190.1278600269179004No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTCACAGAGACCA190.1278600269179004No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA190.1278600269179004No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA170.11440107671601615No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC170.11440107671601615No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT170.11440107671601615No Hit
GGTGTTTGGGATGGAATGTTGATCCACGCAGGTGGTTCGCAGAAGCAGCA170.11440107671601615No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA170.11440107671601615No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGCTCGCAGAAGCAGCA170.11440107671601615No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA170.11440107671601615No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA160.10767160161507401No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG160.10767160161507401No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.10767160161507401No Hit
GGTGTTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCA150.1009421265141319No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG150.1009421265141319No Hit
GGTGTTTGGGATGGAATGGTGTTCCACGCAGGTGGTTCGCAGAAGCAGCA150.1009421265141319No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA150.1009421265141319No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC150.1009421265141319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)