FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15848
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT886155.91241797072186No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA200212.632508833922262No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT9455.9628975265017665No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA3672.3157496214033313No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1841.1610297829379101No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1641.0348308934881372No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT1020.6436143361938416No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC990.6246845027763755No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA820.5174154467440686Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT770.48586572438162545No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT580.36597677940434126No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA420.26501766784452296No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT420.26501766784452296No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA420.26501766784452296No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG370.23346794548207975No Hit
CAACCAAAAGAGAAGGAGGCACCGTCCCAGCCGACCTTGAGGCTGCCGCC370.23346794548207975No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT360.2271580010095911No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT360.2271580010095911No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT350.2208480565371025No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT330.20822816759212517No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT330.20822816759212517No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.18298838970217063No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT290.18298838970217063No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAACAAAGA280.17667844522968199No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA280.17667844522968199No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG280.17667844522968199No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC280.17667844522968199No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.17036850075719334No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG270.17036850075719334Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.17036850075719334No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT240.1514386673397274No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT230.14512872286723877No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.14512872286723877No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA230.14512872286723877No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT220.13881877839475013No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT210.13250883392226148No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT210.13250883392226148No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCCATGTTGT190.11988894497728421No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT190.11988894497728421No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT190.11988894497728421No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG190.11988894497728421No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA180.11357900050479555No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA180.11357900050479555Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG170.10726905603230692No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT170.10726905603230692No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT160.10095911155981827No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT160.10095911155981827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)