FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15898
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA546134.350232733677196No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA522232.8468989810039No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA9245.812051830418921No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC4622.9060259152094603No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA4192.6355516417159395No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT2161.3586614668511763No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA1731.088187193357655No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA980.6164297395898856No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG910.5723990439048937No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG840.5283683482199019No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC700.4403069568499183No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG550.34595546609636435No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG520.3270851679456535No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG500.314504969178513No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC500.314504969178513No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAA460.28934457164423194No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG390.24531387595924017No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTCACAG320.20128318027424832No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC280.1761227827399673No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.16354258397282675No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG250.1572524845892565No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC250.1572524845892565No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC240.15096238520568625No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA240.15096238520568625No Hit
GTGCTGTCTTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC230.14467228582211597No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.13838218643854572No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA210.13209208705497547No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA210.13209208705497547No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.1258019876714052No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC200.1258019876714052No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA190.11951188828783495No Hit
GTGCTGTCTCTCTTTGTGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC190.11951188828783495No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA170.10693168952069443No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG170.10693168952069443No Hit
GTGCTGTCTCTCTTTGATGGAACAGCTGGCTCTCGAGCAGGCATGCCTCA170.10693168952069443No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC170.10693168952069443No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.10693168952069443No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAA160.10064159013712416No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)